Magnetic resonance imaging tracing of superparamagnetic iron oxide nanoparticle-labeled mesenchymal stromal cells for repairing spinal cord injuryOA
Mesenchymal stromal cell transplantation is an effective and promising approach for treating various systemic and diffuse diseases.However,the biological characteristics of transplanted mesenchymal stromal cells in humans remain unclear,including cell viability,distribution,migration,and fate.Conventional cell tracing methods cannot be used in the clinic.The use of superparamagnetic iron oxide nanoparticles as contrast agents allows for the observation of transplanted cells using magnetic resonance imaging.In 2016,the National Medical Products Administration of China approved a new superparamagnetic iron oxide nanoparticle,Ruicun,for use as a contrast agent in clinical trials.In the present study,an acute hemi-transection spinal cord injury model was established in beagle dogs.The injury was then treated by transplantation of Ruicun-labeled mesenchymal stromal cells.The results indicated that Ruicunlabeled mesenchymal stromal cells repaired damaged spinal cord fibers and partially restored neurological function in animals with acute spinal cord injury.T2*-weighted imaging revealed low signal areas on both sides of the injured spinal cord.The results of quantitative susceptibility mapping with ultrashort echo time sequences indicated that Ruicun-labeled mesenchymal stromal cells persisted stably within the injured spinal cord for over 4 weeks.These findings suggest that magnetic resonance imaging has the potential to effectively track the migration of Ruicun-labeled mesenchymal stromal cells and assess their ability to repair spinal cord injury.
Xiaoli Mai;Yuanyuan Xie;Zhichong Wu;Junting Zou;Jiacheng Du;Yunpeng Shen;Hao Liu;Bo Chen;Mengxia Zhu;Jiong Shi;Yang Chen;Bing Zhang;Zezhang Zhu;Bin Wang;Ning Gu
Department of Radiology,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing,Jiangsu Province,ChinaClinical Stem Cell Center,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing,Jiangsu Province,ChinaDepartment of Spinal Surgery,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing,Jiangsu Province,ChinaDepartment of Radiology,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing,Jiangsu Province,ChinaState Key Laboratory of Bioelectronics and Jiangsu Key Laboratory of Biomaterials and Devices,School of Biological Sciences and Medical Engineering,Southeast University,Nanjing,Jiangsu Province,ChinaLaboratory of Image Science and Technology,Southeast University,Nanjing,Jiangsu Province,ChinaDepartment of Neurosurgery,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing,Jiangsu Province,ChinaInstitute of Materials Science and Devices,School of Materials Science and Engineering,Suzhou University of Science and Technology,Suzhou,Jiangsu Province,ChinaDepartment of Radiology,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing,Jiangsu Province,ChinaDepartment of Pathology,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing,Jiangsu Province,ChinaLaboratory of Image Science and Technology,Southeast University,Nanjing,Jiangsu Province,ChinaDepartment of Radiology,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing,Jiangsu Province,ChinaDepartment of Spinal Surgery,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing,Jiangsu Province,ChinaClinical Stem Cell Center,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing,Jiangsu Province,ChinaNanjing Key Laboratory for Cardiovascular Information and Health Engineering Medicine,Institute of Clinical Medicine,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing,Jiangsu Province,China
医药卫生
acute spinal cord injurydiffusion tensor imagingdynamic migrationmesenchymal stromal cellsneural functionneuronal regenerationquantitative susceptibility mappingrepairabilityruicunsuperparamagnetic iron oxide nanoparticle
《Neural Regeneration Research》 2026 (5)
P.2031-2039,9
supported by the National Key R&D Program of China,Nos.2017YFA0104302(to NG and XM)2017YFA0104304(to BW and ZZ)。
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