波纹唇鱼♀×鞍带石斑鱼♂杂交子一代胚胎发育OA北大核心

This experiment aimed to artificially extract eggs from a female humphead wrasse(Cheilinus undulatus)and the sperm from a male giant grouper(Epinephelus lanceolatus),conduct artificial fertilization,and observe as well as describe the development process and characteristics of the hybrid embryos.The characteristics of each develop-mental stage were observed and recorded using an optical microscope.The study found that at a water temperature of 27.5℃±0.5℃,the diameter of the fertilized eggs was 574±31 μm;hatching took 26 h and 21 min to start.The em-bryonic developmental characteristics of the hybrid embryos were the same as those of other bony fish species.The embryonic development process was divided into seven stages:fertilized egg,cleavage,blastula,gastrula,neurula,organ formation,and hatching membrane.The mid-late stage of the original intestine embryo overlapped significantly with the early-middle stage of the neural embryo,causing confusion and making it difficult to distinguish.These study results indicate that the developmental time of the hybrid embryos was longer,deviating from the normal develop-mental time pattern related to diameter and size,which is unique.The developmental time of the hybrid embryos was longer than those of the parent species,indicating an extension influenced by hybridization.It is more similar to that of the male parent,suggesting a marked influence of the male parent on the developmental speed.