基于网络药理学青红活血汤治疗慢性萎缩性胃炎的机制研究OA

Objective To investigate the network pharmacological mechanism of Qinghong Huoxue Decoction,an experienced prescription developed by Hu Bin,a renowned Chinese medicine practitioner,for the treatment of chronic atrophic gastritis.Methods Potential targets of Qinghong Huoxue Decoction were predicted and targets associated with chronic atrophic gastritis were screened using a database.The database was constructed to map the protein-protein interaction network and the topological network,followed by enrichment analysis.Results Based on the Traditional Chinese Medicine System Pharmacology Database and Analysis Platform,compounds were retrieved as follows:68 for chicken blood vine,38 for coix seed,139 for Magnolia officinalis,47 for citron,and 25 for Pseudostellaria heterophylla.Additionally,literature searches identified 86 compounds for radix tetrastigme,110 for Sargent gloryvine,and 29 for Isodon amethystoides.A total of 71 compounds were screened based on the set screening conditions of oral bioavailability≥30%and drug like≥0.18.732 targets related to chronic atrophic gastritis were screened using GeneCards.The mapping relationship between drug targets and chronic atrophic gastritis targets was constructed using the Venn online analysis platform,resulting in 129 targets identified for the treatment of chronic atrophic gastritis with Qinghong Huoxue Decoction.Gene ontology analysis indicated that the mechanism of action of Qinghong Huoxue Decoction for the treatment of chronic atrophic gastritis seemed to be related to the negative regulation of miRNA maturation,positive transcriptional regulation of the RNA polymeraseⅡpromoter involved in cellular response to chemical stimuli,and the mechanism of cell apoptosis,etc.Pathway analysis revealed that the primary pathways affected by Qinghong Huoxue Decoction in the treatment of chronic atrophic gastritis included lipid and atherosclerosis,measles,hypoxia-inducible factor-1(HIF-1)signaling pathways,and advanced glycosylation end products and their receptor(AGE-RAGE)signaling pathway in diabetic complications.Conclusion The treatment of chronic atrophic gastritis with Qinghong Huoxue Decoction may be associated with the negative regulation of miRNA maturation,positive transcriptional regulation of the RNA polymerase Ⅱ promoter involved in cellular responses to chemical stimuli,and the mechanism of cell apoptosis,etc.The primary pathways identified include lipid and atherosclerosis,measles,HIF-1 signaling pathways,and the AGE-RAGE signaling pathway in diabetic complications.