不同剂量卵清蛋白诱导支气管哮喘小鼠模型探讨OA

Objective To investigate the methods of creating a mouse model of bronchial asthma and the optimal sensitization dose of ovalbumin(OVA).Methods Thirty BALB/c mice were randomly divided into the control group,the model group 1(OVA 10 μg,sensitization 2 times),the model group 2(OVA 20 μg,sensitization 2 times),the model group 3(OVA 20 μg,sensitization 3 times),and the model group 4(OVA 50 μg,sensitization 3 times).The serum levels of interleukin(IL)-4,IL-5,IL-13,interferon(IFN)-γ and IgE in mice were detected to evaluate the pathological changes in lung tissue.Results Compared with the control group,the levels of IL-4,IL-5,IL-13,and IgE in the serum of the model groups 1,2,3 and 4 were significantly increased,while the levels of IFN-γ were significantly decreased(P<0.05).Inflammatory cell infiltration was observed in the lung pathology,and the tracheal wall was significantly thickened,and the lumen was narrowed.In them,the model group 1 showed the most significant changes in IL-4 and IL-13,and the model group 3 showed the most significant changes in IFN-γ and IgE.The pathological changes in lung tissue were most pronounced in the model group 3.Conclusion 4 doses of OVA sensitization can establish mice model of bronchial asthma.OVA 20 μg/time and sensitization 3 times may be the most suitable dose for modeling in this experiment.