镁离子体外促进巨噬细胞极化的作用研究OA

Objective To investigate the effects of different concentrations of magnesium ions on the proliferation and polarization of macrophages.Methods Macrophages were cultured in media with different concentrations of magnesium ions.The proliferation of macrophages in each group was detected by cell counting kit-8(CCK8),and the polarization state of macrophages in each group was detected by flow cytometry.The expression levels of polarization-related genes in macrophages of each group were evaluated by real-time quantitative polymerase chain reaction(RT-qPCR).Results MgCl2 at 50,100,and 200 μg/ml had a significant pro-proliferative effect on macrophages(P<0.05),and MgCl2 at 300,400,and 500 μg/ml had a significant inhibitory effect on the proliferation and differentiation of macrophages(P<0.05).Meanwhile,50,100,and 200 μg/ml MgCl2 could significantly promote the polarization of macrophages to M2 type as well as promote the expression of polarization-related genes(P<0.05),and 300 and 400 μg/ml MgCl2 promoted the polarization of macrophages to M1 type(P<0.05).Conclusion 50,100,and 200μg/ml MgCl2 could significantly promote the proliferation and differentiation of macrophages and their polarization to M2 type,while 300 and 400 μg/ml MgCl2 could inhibit the proliferation and differentiation of macrophages and promote their polarization to M1 type.