现场快速筛查即食海参中副溶血弧菌污染的方法OA

In this study,based on an electronic microbial growth analyzer,a simple and rapid method for the quantita-tive screening of viable Vibrio parahaemolyticus.contamination in ready-to-eat sea cucumber products was devel-oped.The liquid companion was sampled from the package of ready-to-eat sea cucumber product,and was directly transferred into a test tube,in which TCBS broth was preloaded.The test tube was then positioned in the electronic microbial growth analyzer to determine the bacterial growth curve,growth rate curve and the time required to reach the maximum growth rate(Tmgr).Using the QC strain as a model Vibrio parahaemolyticus,there was linear relationship between the logarithmic value of viable bacterial concentration(log C)and Tmgr over the range of 5×101～5×105 CFU/mL,with a detection limit of 101 CFU/mL.The regression equation was Tmgr(min)=-48.822 log C(CFU/mL)+443.05(r=0.999 2).When Vibrio parahaemolyticus contamination reached 5.0×104 CFU/mL,the recovery rates were 89.37%to 96.06%.The total time consumption,from the unwrapping of the package of ready-to-eat sea cucumber product to the readout of bacterial concentration,was no more than 4 h.This new method re-quires only a portable instrument and three simple manual steps,making it suitable for on-site analysis.The pro-posed method represents a promising alternative for the on-site screening and quantification of viable Vibrio spp.in ready-to-eat sea cucumber products.[Chinese Fishery Quality and Standards,2025,15(1):10-18].