Plasma dynamic metabolomics during anti-tuberculosis treatment identifies an amino acid panel with diagnostic potentialOA
Tuberculosis(TB)belongs to infectious diseases leading to the high mortality and morbidity worldwide.Since long-term bacilli burden leads to metabolic disorders in TB patients,metabolic biomarkers with diagnosis and prognosis potential are worthy of elucidation.In this study,^(1)H nuclear magnetic resonance spectroscopy(^(1)H NMR)-based plasma metabolomics were investigated dynamically in onset TB patients undergoing conventional anti-TB chemotherapy before and along with the treatment.We found that metabolomic profiles altered before and after the treatment of 2 and 4 months,among which four amino acids,including 4-aminobenzoate,phenylalanine,serine,and threonine,were screened out with significant decrease at 6 months after anti-TB therapy in the training longitudinal samples and validated in another longitudinal samples.Moreover,we have also confirmed the increase of 4 amino acid contents in the periphery of active TB patients when compared to those in healthy controls(HCs).Receiver operating characteristic(ROC)analysis revealed that the combination of 4 amino acids was able to distinguish TB patients from HC with an area under the curve(AUC)value of 0.9120.031(P<0.0001).Therefore,our study has identified an amino acid panel with increased levels in active TB patients and declined along with conventional anti-TB treatment,which might be potential in distinguishing TB patients from HCs as well as prognosis candidates in clinics.
Yong Li;Cheng-cheng Yao;Ping Ji;Hui-yu Wang;Shu-jun Wang;Ying Wang;Qi-jian Cheng;Lu Xia;Ying-ying Chen
Shanghai Institute of Immunology,Department of Microbiology and Immunology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,ChinaShanghai Institute of Immunology,Department of Microbiology and Immunology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China Shanghai Institute of Virology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,ChinaShanghai Institute of Immunology,Department of Microbiology and Immunology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,ChinaShanghai Institute of Immunology,Department of Microbiology and Immunology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,ChinaShanghai Institute of Immunology,Department of Microbiology and Immunology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,ChinaShanghai Institute of Immunology,Department of Microbiology and Immunology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China Shanghai Institute of Virology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China Shanghai Key Laboratory of Emergency Prevention,Diagnosis and Treatment of Respiratory Infectious Diseases,Shanghai 200025,ChinaDepartment of Respiratory Diseases,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,ChinaDepartment of Tuberculosis,Shanghai Public Health Clinical Center,Shanghai 201508,ChinaShanghai Institute of Immunology,Department of Microbiology and Immunology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China
医药卫生
TuberculosisAnti-tuberculosis therapyMetabolomicsAmino acid metabolitesBiomarker
《LabMed Discovery》 2025 (1)
P.7-16,10
supported by grants from the National Key Research and Development Program of China(No.2021YFC2301500)Chinese National Mega Science and Technology Program on Infectious Diseases(Nos.2018ZX10731301-001-004 and 2018ZX10302301-002-002)Shanghai 2020“Science and Technology Innovation Action Plan”Technological Innovation Fund of China(No.20Z11900500)Science Foundation of Shanghai Municipal Commission of Science and Technology,China(No.24ZR1442000)Postdoctoral Fellowship Program of CPSF of China(No.GZC20241041),National Natural Science Foundation of China(No.81501361)Innovative Research Team of High-level Local Universities in Shanghai,China(No.SSMU-ZLCX20180202)Shanghai Frontiers Science Center of Cellular Homeostasis and Human Diseases,China。
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