Dynamic alterations in bacterial and fungal microbiome and inflammatory cytokines following SRV-8 infection in cynomolgus monkeysOACSCDCSTPCD
While viral infections can disturb the host gut microbiome,the dynamic alterations in microbial composition following infection remain poorly characterized.This study identified SRV-8-infected monkeys and classified them into five groups based on infection progression.16S rRNA amplicon sequencing revealed significant alterations in the relative and inferred absolute abundance of bacterial genera UCG-002,Agathobacter,Coprococcus,and Holdemanella during the early stage of SRV-8 infection,coinciding with provirus formation.These microbial shifts were accompanied by functional modifications in bacterial communities at the same stage.In contrast,ITS amplicon sequencing indicated no significant differences in fungal composition between healthy wild-type and SRV-8-infected monkeys.Spearman correlation analyses demonstrated close interactions between intestinal bacteria and fungi following SRV-8 infection.Additionally,SRV-8 seropositive groups exhibited significantly elevated mRNA expression levels of pro-inflammatory(TNF-α,IFN-γ,IL-1β,and IL-6)and anti-inflammatory(IL-10)cytokine genes,highlighting close associations between inflammatory cytokines and immune responses.Overall,these findings provide a comprehensive characterization of bacterial and fungal microbiota dynamics and inflammatory cytokine responses associated with SRV-8 infection,clarifying the pathobiological mechanisms underlying SRV-8 infection from the perspective of the gut microbiome.
Yun-Peng Yang;Li-Bing Xu;Yong Lu;Jing Wang;Yan-Hong Nie;Qiang Sun
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China Institute of Comparative Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China Shanghai Center for Brain Science and Brain-Inspired Technology,Shanghai 201602,China Institute of Neuroscience,CAS Key Laboratory of Primate Neurobiology,State Key Laboratory of Neuroscience,CAS Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences,Shanghai 200031,ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China Institute of Comparative Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,ChinaInstitute of Neuroscience,CAS Key Laboratory of Primate Neurobiology,State Key Laboratory of Neuroscience,CAS Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences,Shanghai 200031,ChinaInstitute of Neuroscience,CAS Key Laboratory of Primate Neurobiology,State Key Laboratory of Neuroscience,CAS Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences,Shanghai 200031,ChinaShanghai Center for Brain Science and Brain-Inspired Technology,Shanghai 201602,China Institute of Neuroscience,CAS Key Laboratory of Primate Neurobiology,State Key Laboratory of Neuroscience,CAS Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences,Shanghai 200031,ChinaShanghai Center for Brain Science and Brain-Inspired Technology,Shanghai 201602,China Key Laboratory of Genetic Evolution&Animal Models,Chinese Academy of Sciences,Kunming,Yunnan 650201,China
医药卫生
Cynomolgus monkeysSRV-8 infectionDynamic alterationsBacterial and fungal microbesInflammatory cytokines
《Zoological Research》 2025 (2)
P.325-338,14
supported by the National Science and Technology Innovation 2030 Major Program(2021ZD0200900)National Key Research and Development Program of China(2022YFF0710901)National Natural Science Foundation of China(82021001,31825018)Biological Resources Program of the Chinese Academy of Sciences(KFJBRP-005)111 Project D18007a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
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