Pathogenic mechanisms of Enterocytozoon hepatopenaei through the parasite-gut microbiome-shrimp(Litopenaeus vannamei)physiology axisOACSCDCSTPCD
The progressive impact of Enterocytozoon hepatopenaei(EHP)infection on gut microbial function in Litopenaeus vannamei remains poorly understood beyond static comparisons between healthy and infected individuals.To close this knowledge gap,metagenomic sequencing was used to characterize the gut microbiomes of normal,long,medium,and short-sized adult shrimp categorized by increasing severity of infection.EHP infection suppressed digestive activity while inducing immune responses compared with healthy shrimp.Increasing infection severity was associated with a gradual decline in gutα-diversity and an expansion of potential pathogens and virulence factors(VFs).In addition,dysbiosis in gut microbiota composition and function,as well as reduced network stability among differential species,intensified with infection severity.Accordingly,we identified 24 EHP-discriminatory species that contributed an overall 83.3%accuracy in diagnosing infection severity without false negatives.Functional pathway analysis revealed significant suppression of metabolic,degradative,and biosynthetic processes in EHP-infected shrimp compared with healthy controls.Among them,map00630 glyoxylate and dicarboxylate metabolism and map00280 valine,leucine and isoleucine degradation were consistently depleted in infected individuals,thereby impairing their digestive function and anti-inflammatory responses.Additionally,EHP infection diversified VFs directly affecting shrimp gut microbiome.These findings support a conceptual model linking EHP pathogenesis to the parasite-gut microbiome-shrimp physiology axis.
Yang-Ming Lu;Jia-Qi Lu;Qi Zhao;Jiong Chen;Jin-Bo Xiong
State Key Laboratory for Quality and Safety of Agro-Products,School of Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,China Key Laboratory of Aquacultural Biotechnology,Ministry of Education,School of 36 Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,ChinaState Key Laboratory for Quality and Safety of Agro-Products,School of Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,China Key Laboratory of Aquacultural Biotechnology,Ministry of Education,School of 36 Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,ChinaState Key Laboratory for Quality and Safety of Agro-Products,School of Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,China Key Laboratory of Aquacultural Biotechnology,Ministry of Education,School of 36 Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,ChinaState Key Laboratory for Quality and Safety of Agro-Products,School of Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,China Key Laboratory of Aquacultural Biotechnology,Ministry of Education,School of 36 Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,ChinaState Key Laboratory for Quality and Safety of Agro-Products,School of Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,China Key Laboratory of Aquacultural Biotechnology,Ministry of Education,School of 36 Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,China Institute of One Health,School of Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,China
农业科技
Increasing Enterocytozoon hepatopenaei infectionGut microbiomeMicrobiota dysbiosisEHP-discriminatory speciesVirulence factors
《Zoological Research》 2025 (2)
P.401-413,13
supported by the Key Natural Science Foundation of Zhejiang Province(Z25C030002)National Natural Science Foundation of China(32371596)Science and Technology Innovation Yongjiang 2035 Key Research and Development Project of Ningbo(2024Z279)One Health Interdisciplinary Research Project(HZ202404)K.C.Wong Magna Fund in Ningbo University。
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