A 6.49-Mb inversion associated with the purple embryo spot trait in potatoOA
The embryo spot trait leads to a deep purple or reddish coloration at the base of the cotyledons of the embryo,visible on both sides of flat potato(Solanum tuberosum)seeds.This trait has long been used by potato researchers and breeders as a morphological marker during dihaploid induction.The formation of embryo spots reflects the accumulation of anthocyanins,but the genetic basis of this trait remains unclear.In this study,we mapped the embryo spot trait to a 6.78-Mb region at the end of chromosome 10 using an F2 population derived from a cross between spotted and spotless plants.The recombination rate in the candidate region is severely suppressed,posing challenges for the map-based cloning of the underlying gene and suggesting large-scale rearrangements in this region.A de novo genome assembly of the spotted individual and a comparative genomic analysis to the reference genome of spotless potato revealed a 6.49-Mb inversion present in the spotted plant genome.The left breakpoint of this inversion occurred in the promoter region of an R2R3 MYB transcription factor gene that is highly expressed in the cotyledon base of spotted embryos but is not expressed in that of spotless embryos.This study elucidated the genetic basis for embryo spot formation in potato and provides a foundation for future cloning of the causative gene.
Pei Wang;Lin Cheng;Jun Pan;Lianlian Ma;Xiaojing Hu;Zhong Zhang;Dawei Li;Yanhui Zhu;Shiwei Chang;Pingping Yuan;Philip Kear;Ludivine Lassois;Guangtao Zhu;Sanwen Huang;Hui Du;Chunzhi Zhang
Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China Yunnan Key Laboratory of Potato Biology,The AGISCAAS-YNNU Joint Academy of Potato Sciences,Yunnan Normal University,Kunming 650500,ChinaShenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China National Key Laboratory of Tropical Crop Breeding,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China Plant Genetics and Rhizosphere Processes Laboratory,TERRA Teaching and Research Center,University of Liège,Gembloux Agro-Bio Tech,Gembloux 5030,BelgiumShenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,ChinaShenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,ChinaShenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,ChinaShenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,ChinaShenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,ChinaShenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,ChinaInternational Potato Center(CIP)China Center for Asia Pacific(CCCAP),Beijing 102199,ChinaInternational Potato Center(CIP)China Center for Asia Pacific(CCCAP),Beijing 102199,ChinaInternational Potato Center(CIP)China Center for Asia Pacific(CCCAP),Beijing 102199,ChinaPlant Genetics and Rhizosphere Processes Laboratory,TERRA Teaching and Research Center,University of Liège,Gembloux Agro-Bio Tech,Gembloux 5030,BelgiumYunnan Key Laboratory of Potato Biology,The AGISCAAS-YNNU Joint Academy of Potato Sciences,Yunnan Normal University,Kunming 650500,ChinaShenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China National Key Laboratory of Tropical Crop Breeding,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,ChinaShenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,ChinaShenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China
农业科技
PotatoEmbryo spotAnthocyanin accumulationInvers
《aBIOTECH》 2025 (1)
P.22-32,11
supported by the China National Key Research and Development Program(Grant No.2019YFE0120500 to CZ)the National Natural Science Foundation of China(32372695)the Agricultural Science and Technology Innovation Program(CAASZDRW202101)the China Postdoctoral Science Foundation(2021M693456)the Central government guidance for local scientific and technological development projects(23ZYQJ304)the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202404).
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