纳秒脉冲电场乳腺肿瘤组织消融安全性的临床前动物实验研究OACSTPCD

Objective To use nanosecond pulsed electric nield(nsPEF)for minimally invasive ablation of goat breast tissue,to explore the safety of pulsed electric field ablation for breast tissue.Methods Three adult normal female goats were selected.After local anesthesia,the ultrasound-guided transcutaneous ablation of breast tissue was performed with the electrode needle connected with the nanosecond pulsed tumor ablation system.Changes in vital signs of experimental goat during ablation were observed,blood biochemistry were detected before,after and during follow-up,ultrasound images of mammary ablation sites were collected,and pathological samples were taken to compare the pathological changes of mammary tissue before and after ablation.Results The nsPEF technique was successful in the ablation of breast tissue in goats.No adverse reaction occurred during the operation and no muscle tremors or contractions were observed around the breast during ablation.Ultrasound examination found that the ablation effect was significant during the regular follow-up after the operation.There was no significant difference in blood examination before and after operation.The histopathology showed apoptosis and necrosis in the ablated region,and normal tissue morphology in the non-ablated region.Conclusion nsPEF technique is effective and safe for breast ablation in goats,and the minimally invasive ablation operation on large preclinical animals is feasible.