人参固本口服液对佐剂关节炎大鼠的抗炎作用研究OA

Objective To investigate the mechanism of anti-inflammatory and protective effects of Renshen Guben Oral Liquid on rats with adjuvant arthritis(AA).Methods An AA rat model was established with the blank group(Group K),the methotrexate group(Group J),the Renshen Guben Oral Liquid low-concentration group(Group R1),the Renshen Guben Oral Liquid high-concentration group(Group R2),and the Renshen Guben Oral Liquid+methotrexate combination group(Group R3),respectively,with 8 rats in each group.These rats were given the drug intervention for 21 days,respectively.At the time of modeling and on Days 7,14,21 and 28,the circumference of the left ankle joint of the rats was measured and scored by arthritis index.The serum levels of interleukin-1β(IL-1β),interleuki-6(IL-6),interleukin-17(IL-17),vascular endothelial growth factor(VEGF),and tumor necrosis factor α(TNF-α)were detected by ELISA.Results After 21 days of treatment,the arthritis index score and the circumference of the left ankle joint of AA rats were lower in groups J,R2 and R3 than those in group M,with statistically significant differences(P＜0.05).The levels of IL-1β,IL-6,IL-17 and VEGF were lower in groups J and R3 than those in group M,with statistically significant differences(P＜0.05).The levels of IL-1β and IL-6 were lower in group R2 than those in group M,with statistically significant differences(P＜0.05).The level of IL-1β was lower in group R1 than that in group M,with a statistically significant difference(P＜0.05).Conclusion Renshen Guben Oral Liquid has certain therapeutic and protective effects on AA rats,which may be related to the reduction of the levels of inflammatory factors,such as IL-1β,IL-6,IL-17,VEGF and TNF-α,and is closely related to the dose and the co-administration of methotrexate.Co-administration of the two drugs may have complementary pharmacological activities on the joint protection and anti-inflammatory regulation effects in AA rats.