米曲霉菌和枯草芽孢杆菌混合菌剂对致病性微生物的影响OA北大核心CSTPCD

Composting is an effective method for the recycling and utilization of cattle manure,and is a key link in achieving planting and resource utilization.In order to investigate the effects of compound microbial agents of Aspergillus oryzae and Bacillus subtilis on the composting of beef cattle manure and pathogenic prokaryotic and eukaryotic microorganisms,this study adopted a strip pile composting fermentation process,using beef cattle manure fermented with compound microbial agents as the research material.The composting lasted for 40 days,and the temperature,pH,C/N,total phosphorus,total potassium,crude fi-ber,ammonium nitrogen of the materials during the composting process were monitored.The dynamic changes of nitrate nitrogen and prokaryotic and eukaryotic microorganisms that are pathogenic to beef cat-tle were detected.The results showed that the inoculation of composite microbial agents entered a high-temperature period(＞50 ℃)on the 7th day,with a maximum temperature of 58.1 ℃ and a high tempera-ture lasting for 9 days.At the end of composting,the pH was 8.63 and the C/N decreased to 13.6;The total phosphorus and total potassium showed a gradual decreasing trend;The contents of ammonium nitrogen and nitrate nitrogen significantly increased;The OPG content of parasitic oocysts decreased from 1 200 to 0;The content of Escherichia coli varies from 4×108 CFU/mL to 0CFU/mL.Staphylococcus,actinomy-cetes,Klebsiella pneumoniae,Proteobacteria,Acinetobacter,Bacillus,Streptococcus,and other prokaryotic microorganisms;Entamoeba,Cryptosporidium,Eimeria,Candida,Aspergillus and other eukaryotic mi-croorganisms have been completely killed or significantly reduced.In summary,the composite microbial a-gent not only has a good effect on the fermentation and composting of beef manure,but also can effectively reduce or kill harmful microorganisms in beef cattle manure.