HPLC-MS/MS测定保健食品中6种大麻素OA

Objective:Qualitative and quantitative analysis of six cannabinoids in health food using high-performance liquid chromatography tandem triple quadrupole mass spectrometry(HPLC-MS/MS).Methods:HLB solid phase extraction column purification,using XBridge C18 chromatography column,with acetonitrile solution as the organic phase and 10 mmol·L-1 ammonium acetate aqueous solution as the aqueous phase.Two ions modes were scanned simultaneously and isotope internal standard quantification was performed.Results:The measured Cannabinoids between 1-100μg·L-1 concentration range shows a good linear relationship,and the correlation coefficients(r2)is greater than 0.999.The detection limit and quantification limit of the method are 5 μg·kg-1 and 10 μg·kg-1.The method has good stability and high sensitivity,and is suitable for qualitative and quantitative analysis of six Cannabinoid in common health products.Conclusion:This study successfully established established a qualitative and quantitative analysis of six cannabinoids in health food,providing technical support for quality evaluation,safety supervision,and risk prevention of health food.