舒巴坦对局灶性脑缺血再灌注大鼠脑组织损伤的影响OACSTPCD
Effect of sulbactam on brain tissue injury in rats with focal cerebral ischemia-reperfusion
目的 探讨舒巴坦对局灶性脑缺血再灌注大鼠脑组织损伤的影响.方法 选取雄性SD大鼠30只,随机分为假手术组、模型组和舒巴坦组,每组10只.采用线栓法制备右侧脑缺血再灌注模型.脑缺血后30 min,模型组腹腔注射10μL的生理盐水,舒巴坦组腹腔注射10 μL的舒巴坦,假手术组不给予任何干预.采用Longa评分评估大鼠的神经功能,Image-ProExpress图像分析脑梗死容积,免疫组化染色检测血清胶质细胞谷氨酸转运体-1(GLT-1)水平.术后30 d接受Morris水迷宫检测学习记忆功能,比较第1、2、3、4、5天的逃避潜伏期.结果 假手术组神经功能评分和脑梗死容积为0,舒巴坦组神经功能评分、脑梗死容积明显低于模型组,差异有统计学意义(P＜0.05);3组大鼠的血清GLT-1水平比较,差异有统计学意义(P＜0.05),其中舒巴坦组和模型组的血清GLT-1明显高于假手术组,而舒巴坦组明显低于模型组,差异均有统计学意义(P＜0.05).检测第1天,3组大鼠的逃避潜伏期比较,差异无统计学意义(P＞0.05);检测第2～5天,3组大鼠的逃避潜伏期比较,差异均有统计学意义(P＜0.05),其中舒巴坦组和模型组的逃避潜伏期明显高于假手术组,舒巴坦组明显低于模型组,差异均有统计学意义(P＜0.05).结论 舒巴坦可有效改善局灶性脑缺血再灌注大鼠的神经功能、学习记忆功能,减少脑梗死容积,降低血清GLT-1水平.
Objective To explore the efect of sulbactam on brain tissue injury in rats with focal cerebral ischemia-reperfusion.Methods Thirty male SD rats were selected and randomly divided into a sham group,a model group and a sulbactam group,with 10 rats in each group.The right cerebral ischemia-reperfusion model was prepared by thread embolism method.Thirty minutes after cerebral ischemia,the model group was intraperitoneally injected with 10 μL normal saline,the sulbactam group was intraperitoneally injected with 10 µL sulbactam,and the sham group was not given any intervention.Longa score was used to evaluate the neurological function of rats,Image-ProExpress imaging was used to analyze cerebral infarction volume,and immunohistochemical staining was used to detect serum glial glutamate transporter-1(GLT-1)levels.Thirty days after surgery,Morris water maze was used to test learning and memory function,and the escape latency on days 1,2,3,4,and 5 was compared.Results The neurological function score and cerebral infarction volume in the sham group were 0,while those in the sulbactam group were significantly lower than those in the model group(P＜0.05).The serum GLT-1 levels of the three groups were statistically significant(P＜0.05),the serum GLT-1 levels of the sulbactam group and the model group were significantly higher than those of the sham group,while the sulbactam group was significantly lower than the model group(P＜0.05).On the first day of test,the escape latency of the three groups was not statistically significant(P＞0.05).On the 2nd to 5th day of testing,the escape latency of the three groups was statistically significant(P＜0.05),the escape latency of the sulbactam group and the model group was significantly higher than that of the sham group(P＜0.05),and the escape latency of the sulbactam group was significantly lower than that of the model group(P＜0.05).Conclusion Sulbactam can effectively improve nerve function,learning and memory function,reduce cerebral infarction volume and serum GLT-1 level in rats with focal cerebral ischemia-reperfusion.
崔鑫;张鹏;古同男;赵艳芝
101300 北京,首都医科大学燕京医学院临床医学系101300 北京,首都医科大学燕京医学院临床医学系101300 北京,首都医科大学燕京医学院临床医学系101300 北京,首都医科大学燕京医学院临床医学系
舒巴坦局灶性脑缺血缺血再灌注神经功能脑梗死容积学习记忆功能胶质细胞谷氨酸转运体-1
SulbactamFocal cerebral ischemiaIschemia reperfusionNeurological functionCerebral infarction volumeLearning and memory functionGlial glutamate transporter-1
《中华神经创伤外科电子杂志》 2023 (6)
333-337,5
Scientific Research Training Fund of Yanjing Medical College in 2020(20kyqd02)Research Project of China Association of Ethnic Medicine(2022Z1041-570221)燕京医学院2020年科研培育基金(20kyqd02)中国民族医药学会科研项目(2022Z1041-570221)
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