右美托咪定改善大鼠脑缺血再灌注后脑损伤的研究OACSTPCD

Objective To explore the effect of dexmedetomidine on brain injury after cerebral ischemia reperfusion in rats.Methods A total of 40 male SD rats were selected and randomly divided into Sham group with 10 rats,CI/RI group(cerebral ischemia-reperfusion injury rat model)with 8 rats,DEX group(treated with DEX intervention on the basis of CI/RI group)with 8 rats,and RES group(treated with rosatovir intervention on the basis of CI/RI group as a positive control)with 8 rats after removing dead mice.Modeling was performed using repeated cerebral ischemia-reperfusion surgery.Longa score was used to evaluate neurological function in rats 24 h after modeling;the content of S100 calcium-binding protein B(S100B)in serum and glutamic acid(Glu)and gamma-aminobutyric acid(GABA)in brain tissue were detected by ELISA;and the volume of cerebral infarction was calculated by Image J;the pathological changes of brain were detected by HE method;the protein expression of S100B,cyclooxygenase-2(COX-2)and glycosylation end product receptor(RAGE)in brain tissue was detected by Western blot.Results The Longa scores of rats in the DEX group and RES group were significantly lower than those in the CI/RI group,and the difference was statistically significant(P＜0.05).The serum S100B levels in the DEX group and RES group rats were significantly lower than those in the CI/RI group at 12,24,and 48 h after surgery,with statistical significance(P＜0.05).The volume of cerebral infarction in the DEX group and RES group was significantly lower than that in the CI/RI group,and the difference was statistically significant(P＜0.05).The Glu content in the brain tissue of rats in the DEX and RES groups was significantly reduced compared to the CI/RI group,while the GABA content was significantly increased compared to the CI/RI group,with a statistically significant difference(P＜0.05).The expression of S100B,COX-2,and RAGE proteins in the brain tissue of rats in the DEX and RES groups was significantly reduced compared to the CI/RI group,and the differences were statistically significant(P＜0.05).Conclusion Dexmedetomidine improved neurological function and reduced brain injury in rats after cerebral ischemia-reperfusion,which may be related to the inhibition of RAGE/S100B signaling and COX-2/S100B signaling.