miR-642a-3p对藻蓝色素抑制非小细胞肺癌活性的调控作用OA北大核心CSCDCSTPCDEI

Phycocyanin(PC)is a kind of natural pigment protein complex derived from marine algae.It is also a type of important food functional factor with anti-cancer,anti-inflammatory,and immune-enhancing activities,and has been widely used in functional food,medicine and cosmetics.Phycocyanin has been proven to inhibit the activity of non-small cell lung cancer(NSCLC)cells,but the underlying regulatory mechanism is not fully understood.This study focused on miR-642a-3p,and investigated the potential regulating mechanism of phycocyanin in NSCLC cells.The results showed that phycocyanin upregulated the expression level of miR-642a-3p in four NSCLC cells(A549,H1299,H460 and LTEP-a2).Overexpression of miR-642a-3p levels significantly inhibited the proliferation,migration and unanchored growth of four cells in vitro.In addition,transfection of miRNA inhibitors to interfere with the expression of miR-642a-3p could enhance cell viability to a certain extent.This study preliminarily revealed that the up-regulation expression of miR-642a-3p was involved in the regulation of inhibiting NSCLC cell viabilities by phycocyanin,which could lay a sci-entific theoretical basis for the in-depth study and utilization of phycocyanin.