细菌素纯化技术及其应用研究进展OA

Bacteriocin is a kind of polypeptide synthesized by bacteria in the ribosome and released into the cell,which has antibacterial or bactericidal effect.A large number of studies had shown that bacteriocin had the effects of regulating intestinal microbial population,anti-inflammatory and anticancer.However,the high cost of bacteriocin purification,low yield and some environmental pollution are important factors limiting the wide application of bacteriocin.Improving the purification ratio and recovery rate of bacteriocin is the key to its purification.Increasing the specific activity and yield of bacteriocin is very important for its production and application.Reasonable selection of purification technology can effectively improve the yield,purity and recovery of bacteriocin.Because of the difference in molecular weight and charge-carrying properties of bacteriocins,different purification techniques should be selected according to their characteristics.The purification of bacteriocin includes crude separation purification and fine separation purification.The crude separation purification technology includes ammonium sulfate precipitation method,organic solvent extraction method,macroporous resin method and dialysis method,and the fine separation and purification technology includes ion exchange chromatography,gel filtration chromatography,reverse phase high performance liquid chromatography and so on.The widely used bacteriocin purification technologies include ammonium sulfate precipitation,ion exchange chromatography,gel filtration chromatography and reversed phase high performance liquid chromatography,etc.,and the two-step and three-step purification technologies combined by these technologies have been widely used in recent years.In recent years,non-traditional purification techniques such as fast protein liquid chromatography had been gradually applied to the fine separation and purification of bacteriocins.In this paper,the purification technology and application of bacteriocin were summarized and analyzed,which could provide reference for further development and application of bacteriocin.The purification technology of bacteriocin with high efficiency,low cost,no pollution and green environmental protection is the key to its further research,and the breakthrough of these technical difficulties will lay the foundation for the application of bacteriocin in the medical field.