Reconstitution of double-negative T cells after cord blood transplantation and its predictive value for acute graft-versus-host diseaseOACSTPCDMEDLINE

Background:With an increasing number of patients with hematological malignancies being treated with umbilical cord blood transplantation(UCBT),the correlation between immune reconstitution(IR)after UCBT and graft-versus-host disease(GVHD)has been reported successively,but reports ondouble-negative T(DNT)cell reconstitution and its association with acute GVHD(aGVHD)after UCBT are lacking. Methods:A population-based observational studywas conducted among 131 patients with hematological malignancies who underwent single-unit UCBT as their first transplant at the Department of Hematology,the First Affiliated Hospital of USTC,between August 2018 and June 2021.IR differences were compared between the patients with and without aGVHD. Results:The absolute number of DNT cells in the healthy Chinese population was 109(70-157)/pL,accounting for 5.82(3.98-8.19)％of lymphocytes.DNT cells showed delayed recovery and could not reach their normal levels even one year after transplantation.Importantly,the absolute numberand percentage of DNT cells were significantly higher in UCBT patients without aGVHD than in those with aGVHD withinone year(F=4.684,P=0.039 and F=5:583,P=0.026,respectively).In addition,the number of DNT cells in the first month-after transplantation decreased significantly with the degree of aGVHD increased,and faster DNT cell reconstitution in the first month after UCBT was an independent protective factor for aGVHD(HR=0.46,95％confidence interval[CI]:0.23-0.93;P=0.031). Conclusions:Compared to the number of DNT cells in Chinese healthy people,the reconstitution of DNT cells in adults with hematological malignancies after UCBT was slow.In addition,the faster reconstitution of DNT cells in the early stage after transplantation was associated with a lower incidence of aGVHD.