CELSR2基因启动子区甲基化与代谢综合征痰证的关系研究OA北大核心CSTPCD

Objective To detect the DNA methylation level of CpG sites in CELSR2 gene promoter region in patients with phlegm syndrome,non-phlegm syndrome of metabolic syndrome(MS)and healthy people,and to explore the epigenetic mechanism of phlegm syndrome of MS.Methods MS patients were collected,and 27 patients with phlegm syndrome and 27 patients without phlegm syndrome were screened by syndrome differentiation method,and 27 healthy people were selected as healthy group.Physicochemical indexes were detected,including systolic blood pressure(SBP),diastolic blood pressure(DBP),body mass index(BMI),waist circumference(WC),fasting plasma glucose(FPG),triglyceride(TG),high density lipoprotein cholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C).The DNA methylation level of 41 CpG sites in the promoter region of CELSR2 gene was detected by MethylTarget method.Results Compared with the healthy group,the SBP,DBP,BMI,WC,FPG and TG increased and the HDL-C decreased in phlegm syndrome and non-phlegm syndrome group(P＜0.01).Compared with the non-phlegm syndrome group,the DBP,BMI,WC,FPG and TG increased in phlegm syndrome group(P＜0.05).Compared with the healthy group,the DNA methylation levels of CpG28 and CpG34 in the non-phlegm syndrome group decreased(P＜0.05),and the DNA methylation levels of CpG24 and CpG34 in the phlegm syndrome group decreased(P＜0.05).Compared with the non-phlegm syndrome group,the DNA methylation levels of CpG20,CpG24 and CpG34 in the phlegm syndrome group decreased(P＜0.01).Compared with the healthy group,the number of smoking and drinking in MS patients was higher(P＜0.01).Compared with the non-phlegm syndrome group,the number of drinkers in the phlegm syndrome group increased(P＜0.05).Compared with the normal methylation rate of CELSR2 gene CpG34,the risk of MS phlegm syndrome with low methylation was 4.14 times(95％CI:1.25-13.70,P＜0.05).Compared with no drinking,the risk of MS phlegm syndrome with drinking was 3.55 times(95％CI:1.07-11.82,P＜0.05).Conclusions The increase of DBP,BMI,WC,FPG,TG may increase the risk of MS phlegm syndrome.The DNA hypomethylation of CELSR2 gene CpG34 locus may be the epigenetic basis of MS phlegm syndrome.Drinking is a risk factor for the formation of MS phlegm syndrome.