目的 研究胎盘 11β-羟基类固醇脱氢酶(11β-hydroxysteroid dehydrogenase,11β-HSD)的表达差异与双胎生长不一致的关系.方法 选取 2021 年 1 月至 2022 年 12 月在嘉兴市妇幼保健分娩的双绒毛膜双胎 27 对,根据双胎出生体质量差是否在 20%及以上,分为双胎生长不一致(discordant twins,DT)组(实验组)及双胎生长一致(concordant twins,CT)组(对照组).采用qRT-PCR法检测两组胎儿胎盘组织中 11β-HSD1 和 11β-HSD2 mRNA的表达水平及差异.结果 两组胎儿胎盘组织中11β-HSD1 mRNA的表达水平比较,差异无统计学意义(P>0.05);CT组A孩与B孩的11β-HSD2 mRNA表达水平比较,差异无统计学意义(P>0.05),而DT组小孩胎盘 11β-HSD2 mRNA的表达水平显著低于DT组大孩的表达水平,差异有统计学意义(P<0.05).结论 胎盘 11β-HSD2 的表达差异可能与双绒毛膜双胎生长不一致的发生有关.
Objective To study the relationship between the expression difference of 11β-hydroxysteroid dehydrogenase(11β-HSD)in placenta with discordant twins.Methods Twelve pairs of discordant twins(DT)group delivered in Jiaxing Maternal and Child Health Hospital from January 2021 to December 2022 were selected as the experimental group,and 15 pairs of concordant twins(CT)group were selected as the control group.The mRNA expression levels of 11β-HSD1 and 11β-HSD2 in fetal placenta of the two groups were detected by qRT-PCR.Results The mRNA expression of 11β-HSD1 showed no significant difference between two groups(P>0.05).The mRNA expression of 11β-HSD2 did not statistically significantly differ between children A and B in CT group(P>0.05),while the smaller children had a lower mRNA expression of 11β-HSD2 in DT group(P<0.05).Conclusion The difference of placental 11β-HSD2 expression may be related to the occurrence of twin growth discordant.
倪丽;朱晖
嘉兴学院附属医院浙江省嘉兴市妇幼保健院药剂科,浙江嘉兴 314000嘉兴学院附属医院浙江省嘉兴市妇幼保健院产科,浙江嘉兴 314000
临床医学
双胎生长不一致;胎盘;11β-羟基类固醇脱氢酶;表达差异;双绒毛膜双胎
Twin inconsistent growth;Placenta;11β-Hydroxysteroid dehydrogenase;Expression difference;Dichorionic twins
《中国现代医生》 2024 (014)
49-52 / 4
浙江省医药卫生科技计划项目(2021KY1121);浙江省嘉兴市科技计划项目(2021AD30130);浙江省嘉兴市科技计划项目(2023AD31100)
10.3969/j.issn.1673-9701.2024.14.011
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