目的 研究芹糖葛根素对非酒精性脂肪肝(NAFLD)模型小鼠的防治作用,及其对肝脂代谢的影响.方法 将32只雄性C57BL/6J小鼠随机分为正常组、模型组和低、高剂量实验组,每组8只.正常组给予普通维持饲料;其余3组饲养高脂饮食构建NAFLD模型.低、高剂量实验组分别灌胃给予5、10 mg·kg-1芹糖葛根素;正常组与模型组均灌胃给予等量二甲基亚砜.4组小鼠每天给药1次,连续给药8周.观察小鼠体质量变化,用试剂盒检测小鼠血清中谷丙转氨酶(GPT)、谷草转氨酶(GOT)含量和肝组织中总胆固醇(TC)、三酰甘油(TG)含量,用蛋白质印迹法检测乙酰辅酶A羧化酶1(ACC1)和磷酸化5'-磷酸腺苷活化蛋白激酶(p-AMPK)蛋白的表达水平.结果 正常组、模型组和低、高剂量实验组的体质量分别为(32.69±1.37)、(45.51±3.29)、(41.18±3.22)和(38.28±2.62)g,血清 GPT 分别为(36.53±6.44)、(134.56±39.91)、(121.54±43.38)和(75.92±25.98)U·L-1,小鼠血清 GOT 分别为(60.81±8.74)、(188.51±39.70)、(156.02±41.67)和(126.79±16.49)U·L-1,肝 TC 分别为(2.17±0.24)、(6.46±1.22)、(5.86±0.66)和(3.62±0.45)mmol·L-1,肝 TG 分别为(0.57±0.09)、(1.39±0.27)、(1.28±0.34)和(0.73±0.19)mmol·L-1,ACC1蛋白相对表达水平分别为1.01±0.04、2.00±0.02、1.47±0.08 和 1.20±0.09,p-AMPK 蛋白相对表达水平分别为 1.03±0.09、0.33±0.02、0.66±0.04 和 0.95±0.08.模型组的上述指标与高剂量实验组和正常组相比较,在统计学上差异均有统计学意义(均P＜0.05).结论 芹糖葛根素通过调控AMPK信号通路,从而改善NAFLD小鼠的脂代谢.
Objective To investigate the preventive effects of puerarin apioside on a non-alcoholic fatty liver disease(NAFLD)mouse model and its influence on hepatic lipid metabolism.Methods Thirty-two male C57BL/6J mice were randomly divided into the normal group,model group and experimental-L,-H groups,with 8 mice in each group.The normal group was given regular maintenance feed;the other 3 groups were fed to construct NAFLD model.Experimental-L,-H groups were given 5 and 10 mg·kg-1 puerarin apioside by gavage.Normal and model groups were treated with an equal amount of dimethylsulfoxide by gavage.Four groups were administered once a day for 8 weeks.Changes in mouse body weight were measured.Serum levels of glutamic-pyruvic transaminase(GPT)and glutamic-oxaloacetic transaminase(GOT)and the levels of total cholesterol(TC)and triglycerides(TG)in hepatic tissue were determined by kit.Western blot was used to evaluate the expression levels of acetyl-coenzyme A carboxylase 1(ACC1)and phosphorylated 5'-adenosine monophosphate-activated protein kinase(p-AMPK).Results The body weights of mice in the normal,model and experimental-L,-H groups were(32.69±1.37),(45.51±3.29),(41.18±3.22)and(38.28±2.62)g;serum GPT levels were(36.53±6.44),(134.56±39.91),(121.54±43.38)and(75.92±25.98)U·L-1;serum GOT levels were(60.81±8.74),(188.51±39.70),(156.02±41.67)and(126.79±16.49)U·L-1;hepatic TC levels were(2.17±0.24),(6.46±1.22),(5.86±0.66)and(3.62±0.45)mmol·L-1;hepatic TG levels were(0.57±0.09),(1.39±0.27),(1.28±0.34)and(0.73±0.19)mmol·L-1;the relative expression levels of ACC1 protein were 1.01±0.04,2.00±0.02,1.47±0.08 and 1.20±0.09;the relative expression levels of p-AMPK protein were 1.03±0.09,0.33±0.02,0.66±0.04 and 0.95±0.08,respectively.The differences between the model group and the normal and experimental-H groups were statistically significant for all the above indicators(all P＜0.05).Conclusion Puerarin apioside can improve the hepatic lipid metabolism in NAFLD mice by regulating the AMPK signaling pathway.
尚志;胡银璐;徐圣麟;林莉莉
上海中医药大学附属曙光医院中西医结合疫病研究所,上海 200021无锡卫生高等职业技术学校 药学系,江苏 无锡 214028天津中医药大学中药学院,天津 301617
中医学
芹糖葛根素;非酒精性脂肪肝;脂代谢;5'-磷酸腺苷活化蛋白激酶信号通路
puerarin apioside;non-alcoholic fatty liver disease;lipid metabolism;5'-adenosine monophosphate activated protein kinase signaling pathway
《中国临床药理学杂志》 2024 (011)
1598-1602 / 5
国家自然科学基金青年科学基金资助项目(82104606)
10.13699/j.cnki.1001-6821.2024.11.012
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