目的 探究粉防己碱上调人第10号染色体缺失的磷酸酶(PTEN)表达对逆转肝细胞癌细胞顺铂耐药的作用.方法 将Huh7/DDP细胞分为空白对照组、NC组、a组、b组.空白对照组不接受任何处理,仅用正常培养基培养;NC组在正常培养基中加入16 μmol·L-1顺铂;a组在正常培养基中加入16 μmol·L-1顺铂,添加5 μmol·L-1粉防己碱;b组在正常培养基中加入16 μmol·L-1顺铂,并添加5 μmol·L-1粉防己碱,同时过表达PTEN.用细胞计数试剂盒-8(CCK-8)法评估细胞存活率,用免疫荧光法检测PTEN基因的表达情况,以及多药耐药相关蛋白(MRP)-3和MRP-5的表达情况.结果 空白对照组、NC组、a组和b组的细胞存活率分别为(98.74±9.26)％、(55.08±6.38)％、(35.17±4.87)％和(24.06±4.16)％,PTEN 表达水平分别为 1.00±0.13、1.03±0.13、1.58±0.17 和 2.18±0.24,MRP-3 蛋白表达水平分别为1.00±0.08、1.03±0.14、0.71±0.07 和0.48±0.04,MRP-5 蛋白表达水平分别为 1.00±0.11、0.97±0.12、0.63±0.07 和 0.40±0.05.空白对照组的上述指标与NC组比较,a组的上述指标与b组比较,在统计学上差异均有统计学意义(均P＜0.05).结论 粉防己碱通过上调PTEN表达来逆转肝细胞癌细胞顺铂耐药的作用,并发现其能抑制耐药相关蛋白MRP-3和MRP-5的表达.
Objective To investigate the effect of tetrandrine on reversing cisplatin resistance in hepatocellular carcinoma cells by up-regulating phosphatase and tensin homolog deleted on chromosome ten(PTEN)expression.Methods Huh7/DDP cells were divided into control group,NC group,group a and group b.The control group received no treatment and was cultured with normal medium only.In NC group,16 μmol·L-1 cisplatin was added into normal medium.In group a,16 μmol·L-1 cisplatin and 5 μmol·L-1 tetrandrine were added into normal medium.In group b,16 μmol·L-1 cisplatin and 5 μmol·L-1 tetrandrine were added to normal medium,and PTEN was overexpressed.Cell survival was assessed by cell counting kit-8(CCK-8)method,and PTEN gene expression was detected by immunofluorescence.The impact on multidrug resistance associated protein(MRP)-3 and MRP-5 were observed.Results The cell survival rates of control group,NC group,a group and b group were(98.74±9.26)％,(55.08±6.38)％,(35.17±4.87)％and(24.06±4.16)％;the expression of PTEN gene were 1.00±0.13,1.03±0.13,1.58±0.17 and 2.18±0.24;the expression of MRP-3 protein were 1.00±0.08,1.03±0.14,0.71±0.07 and 0.48±0.04;MRP-5 protein expression were 1.00±0.11,0.97±0.12,0.63±0.07 and 0.40±0.05,respectively.Compared between NC group and control group,compared between group a and group b,there were statistically significant differences in the above indexes(all P＜0.05).Conclusion Tetrandrine reverses cisplatin resistance in hepatocellular carcinoma cells by up-regulating PTEN expression and inhibits the expression of drug resistance-related proteins MRP-3 and MRP-5.
蒋飞;秦依雯
常州市妇幼保健院药事科,江苏常州 213000
药学
粉防己碱;肝细胞癌;顺铂耐药性;人第10号染色体缺失的磷酸酶
tetrandrine;hepatocellular carcinoma;cisplatin resistance;phosphate and tension homology deleted on chromosome ten gene
《中国临床药理学杂志》 2024 (011)
1565-1568 / 4
10.13699/j.cnki.1001-6821.2024.11.005
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