四氧化三铁(Fe3O4)磁性纳米颗粒因其制备简单,在外加磁场作用下具有靶向性,并且表面易接枝等特性,可作为被动靶向载体应用于基因治疗领域.本研究采用溶剂热法制备纳米颗粒,并调控堆积生长时间,制得粒径在4～9 nm范围内可控的油相Fe3O4纳米颗粒;使用内消旋-2,3-二巯基丁二酸(DMSA)二次取代其表面的油酸分子,使其具备良好的水相分散性;通过酰胺化反应在其表面接枝支链型聚乙烯亚胺(PEI),最终得到Fe3O4-DMSA-PEI磁性纳米颗粒.研究发现,Fe3O4-DMSA-PEI磁性纳米颗粒的表面Zeta电位高达(52.50±1.94)mV,具有一定的超顺磁性(14.48 emu/g,1 emu/g=1 A·m2/kg).磁性纳米颗粒与质粒DNA的质量比为15∶1时可完全阻滞DNA在凝胶上的电泳,装载量高达6.67％.本研究制备的Fe3O4-DMSA-PEI磁性纳米颗粒具有一定的基因负载能力,有望作为基因载体应用于基因转染领域.
Ferroferric oxide(Fe3O4)magnetic nanoparticles are widely used as passive targeting carriers in gene therapy,due to their simple preparation,targeting under external magnetic field and easy surface grafting.This study synthesized oil phase Fe3O4 nanoparticles with controllable particle sizes in the range from 4 to 9 nm by regulating the accumulation growth time in the solvothermal method.Then,meso-2,3-dimercaptosuccinic(DMSA)was employed to double exchange oleic acid molecules on its surface to provide good water dispersibility.Finally,Fe3O4-DMSA-PEI magnetic nanoparticles were obtained by grafting branched polyethylenimine(PEI)onto Fe3O4-DMSA surface through amidization reaction.The results demonstrate that the Fe3O4-DMSA-PEI magnetic nanoparticles have a surface Zeta potential of(52.50±1.94)mV,remaining a certain degree of superparamagnetism(14.48 emu/g,1 emu/g=1 A·m2/kg).When the mass ratio of Fe3O4-DMSA-PEI magnetic nanoparticles to plasmid DNA is 15∶1,it can completely block DNA and its loading capacity is as high as 6.67％.The Fe3O4-DMSA-PEI magnetic nanoparticles prepared in this study have a certain gene delivery ability and are expected to be used as gene carriers in the field of gene transfection.
蔡和庆;韩璐;杨松松;薛新玉;张扣;孙志成;刘儒平;胡堃;危岩
北京印刷学院印刷与包装工程学院,北京 102600清华大学生命有机磷化学及化学生物学教育部重点实验室,北京 100084
四氧化三铁;磁性纳米颗粒;基因载体;表面改性
ferroferric oxide;magnetic nanoparticle;gene carrier;surface modification
《无机材料学报》 2024 (005)
517-524 / 8
国家自然科学基金(61971049,62211530446,22278037);北京印刷学院科研创新团队项目(Ea202205);北京市高等教育学会2023年立项重点课题(ZD202312)National Natural Science Foundation of China(61971049,62211530446,22278037);Scientific Research Innovation Project from Beijing Institute of Graphic Communication(Ea202205);The 2023 Key Project from Beijing Association of Higher Education(ZD202312)
10.15541/jim20230464
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