响应面法优化提取壮药三七姜总酚酸及其镇痛抗炎作用机制研究OA北大核心CSTPCD

Response surface method(RSM)was used to optimize the extraction process of Stahlianthus involucratus total phenolic acids and explore its analgesic and anti-inflammatory mechanism.Total phenolic acids were extracted from Stahlianthus involucratus by reflux extraction.The extraction process was optimized by single factor test and Box-Behnken test,and the effects of ethanol concentration,liquid-to-material ratio,and extraction time on the yield of total phenolic acids were explored.Writhing model and cotton ball granuloma model of mice induced by acetic acid were established to study the analgesic and anti-inflammatory mechanism of total phenolic acids in Stahlianthus involucratus.The results showed that the optimum extraction process of total phenolic acids from Stahlianthus involucratus were an ethanol concentration of 42％,liquid-material ratio of 60 mL/g,and reflux time of 60 min.Under these conditions,the yield of total phenolic acids of Stahlianthus involucratus can reach 6.09 mg/g.Compared with the model group,each dose of ethanol extract of Stahlianthus involucratus had an inhibitory effect on granuloma inflammation,reduced the number of writhing,decreased the contents of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),prostaglandin E2(PGE2),interleukin-1β(IL-1β),5-hydroxytryptamine(5-HT)and increased the content of cyclic adenosine monophosphate(cAMP).The study indicates that the optimized extraction process of Stahlianthus involucratus by response surface methodology is stable and reliable,and can be used for the extraction of total phenolic acids from Stahlianthus involucratus.Stahlianthus involucratus has certain analgesic and anti-inflammatory activities,and its mechanism may be related to reducing the secrete of pro-inflammatory cytokines such as IL-6,TNF-α,IL-1β,and PGE2 in vivo.