目的 探讨柚皮素(Nar)对瘢痕疙瘩成纤维细胞(KFs)增殖、迁移和胶原合成的调控作用和分子机制.方法 将KFs分为对照组和Nar组.对照组常规培养,Nar组以10、25、50、100、150 μmol/L浓度的Nar对细胞进行干预.利用CCK-8实验分析不同浓度下Nar对KFs增殖的影响;细胞划痕实验检测Nar 50 μmol/L组对KFs迁移能力的影响;Western blot检测Nar 10、25、50 μmol/L组对KFs表达Col-1、FN、α-SMA、MMP9蛋白表达水平的影响,以及Nar对TGF-β1诱导后KFs表达Smad2/3、p-Smad2/3蛋白水平的影响.结果 CCK-8实验结果显示,与对照组相比,给予Nar 50 µmol/L以上浓度时,KFs增殖能力受到明显抑制(P＜0.05).细胞划痕实验显示给予Nar 50 µmol/L时,KFs迁移能力受到显著抑制(P＜0.01).Western blot 显示 50 μmol/L Nar 可下调 Col-1、FN、α-SMA、MMP9 蛋白表达,并且可下调TGF-β1诱导后p-Smad2/3蛋白表达(均P＜0.05).结论 Nar可能通过下调TGF-β1/Smad信号通路抑制KFs增殖、迁移和胶原合成.
Objective To explore the effects and molecular mechanisms of naringenin(Nar)on the proliferation,migration and collagen synthesis of keloid fibroblasts(KFs).Methods KFs were divided into control group and Nar group treated with Nar at concentrations of 10,25,50,100 and 150 µmol/L,and the effects of Nar on the proliferation of KFs were analyzed using CCK-8 assay.Western blot was performed to detect the effects of Nar on the expression levels of Col-1,FN,α-SMA,and MMP9,and the expression levels of Smad2/3 and p-Smad2/3 in KFs pretreated with TGF-β1.Results Compared with the control group,the proliferation ability of KFs was sig-nificantly inhibited by Nar at a concentration of ≥50 μmol/L(P＜0.05).Similarly,the migra-tion ability of KFs was significantly inhibited by 50 μmol/L of Nar(P＜0.01).Nar down-regula-ted the expression levels of Col-1,FN,α-SMA and MMP9 proteins in KFs,and p-Smad2/3 pro-teins in TGF-β1-treated KFs(all P＜0.05).Conclusion Nar may inhibit the proliferation,mi-gration and collagen synthesis of KFs by down-regulating the TGF-β1/Smad signaling pathway.
孙佳锐;卢博;李卉欣;元星花;金哲虎
延边大学附属医院,吉林 延吉 133000
瘢痕疙瘩;柚皮素;成纤维细胞;TGF-β1/Smad信号通路
keloid;naringenin;fibroblasts;TGF-β1/Smad signaling pathway
《皮肤性病诊疗学杂志》 2024 (005)
297-302 / 6
国家自然科学基金项目(82260617;81960561)
10.3969/j.issn.1674-8468.2024.05.002
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