铁皮石斛和红龙石斛杂交后代繁育技术研究OACSTPCD

The breeding technology of hybrid offspring of Dendrobium officinale and D.nestor was studied.The results showed that when D.officinale was used as female parent in hybridization,the success rate was higher,reaching 96.7%.The best breeding culture medium was HB01(containing Hyponex No.1 3.0 g)+6-BA 0.5 mg/L+NAA 0.1 mg/L.The best proliferation culture medium was HB01+BA 0.6 mg/L+NAA 0.1 mg/L+50 g/L banana.The best intensified culture medium was HB01+BA 0.3 mg/L+NAA 0.2 mg/L+50 g/L banana.The best rooting culture medium was HB01+NAA 0.1 mg/L+IBA 0.1 mg/L+0.25 g/L peptone.The better transplanting medium for the tissue culture seedlings of filial generation was shavings and the No.5 bark(0.8～1.2 cm in diameter).The seedlings at steady state can be tied with transplant medium in the open air to create tropical garden landscape.The filial generation of the two orchid had strong resistance,and were resistant to the strong light and drought.The delicate buds were suitable for choosing as explants when superior single plant of filial generation was used for propagation.The 0.1%mercuric chloride was used to sterilize the buds for 5 min,and the sprouting culture medium was HB01+6-BA 0.6 mg/L+NAA 0.1 mg/L.