本研究以 CaO、煅烧后的牡蛎壳、蛤蜊壳、扇贝壳和脉红螺壳为原料,分别采用反相微乳液法和聚乙二醇(PEG)辅助微波加热法制备了纳米羟基磷灰石(HA),并从产物的物理化学特性以及形态学参数方面进行比较.利用傅里叶变换红外光谱、X 射线衍射对各组产物进行了化学表征,扫描电子显微镜观察样品的表面形貌,Zeta电位仪测定样品表面的电势差.结果表明,反相微乳液法制备的HA尺寸在(104.10±1.95)nm至(207.90±3.75)nm范围内,为近球形颗粒;而PEG辅助微波加热法制备得到的HA 尺寸在(61.17±3.11)nm 与(182.70±1.05)nm 范围内,倾向于形成椭圆及棒状的颗粒,稳定性更高,且各组样品均表现出完全的亲水性.此外,对比于CaO制备的HA,以贝壳为原料制备的HA具有更好的纳米结构,其中利用脉红螺壳制备的HA在粒径大小、稳定性以及亲水性等方面均具有优势,并且具有更接近天然骨的钙磷比.因此脉红螺壳可以作为PEG辅助微波加热法制备羟基磷灰石的主要原料.
In the present study,we used CaO and calcined oyster,clam,scallop,and snail shells as raw materials to synthesize nanohydroxyapatite(HA)using the reverse-phase microemulsion method and polyethylene glycol(PEG)-assisted microwave heating and compared the physicochemical characteristics and morphological parameters of HA.Fourier transform infrared spectroscopy and X-ray powder diffraction were used to characterize the product,scanning electron microscopy was employed to observe the surface morphology,and a Zeta potential analyzer was utilized for potential determination.The results showed that HA fabricated using the reverse-phase microemulsion method had a particle size between(104.10±1.95)and(207.90±3.75)nm and a sphere-like morphology;however,HA prepared using the PEG-assisted microwave method presented a smaller particle size that varied from(61.17±3.11)to(182.70±1.05)nm and tended to have an oval-like and rod-like shape,while showing better stability and hydrophilicity.In addition,compared with chemically synthesized HA,HA derived from seashells had a better nanostructure,and HA derived from snail shells outperformed others in terms of particle size,stability,and wet-tability.Furthermore,the Ca/P ratio of snail-shell HA was closer to that of the natural bone.Therefore,snail shells can be used as the main raw material for synthesizing HA using the PEG-assisted microwave method.
田璐琦;杨皓月;邢荣娥;刘松;李克成;于华华;李鹏程
中国科学院海洋研究所 实验海洋生物学重点实验室,山东 青岛 266071||中国科学院大学,北京 100049中国科学院海洋研究所 实验海洋生物学重点实验室,山东 青岛 266071||中国科学院海洋大科学研究中心,山东 青岛 266071中国科学院海洋研究所 实验海洋生物学重点实验室,山东 青岛 266071||青岛海洋科学与技术国家实验室 海洋药物与生物制品功能实验室,山东 青岛 266237||中国科学院海洋大科学研究中心,山东 青岛 266071
轻工业
贝壳;反相微乳液法;微波加热;聚乙二醇;羟基磷灰石
sea shells;microemulsion method;microwave heating;polyethylene glycol;hydroxyapatite
《海洋科学》 2024 (003)
95-102 / 8
国家重点研发计划项目(2019YFD0902105);福建省科技计划项目-STS计划(2021T3013);中国科学院海洋大科学研究中心重点部署项目(技术研发类)(COMS2020J04) The National Key Research and Development Program of China,No.2019YFD0902105;Fujian science and technology planning project-STS program,No.2021T3013;Key deployment projects of the Marine Science Research Center of Chinese Academy of Sciences,No.COMS2020J04
10.11759/hykx20230303001
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