益肾活血解毒方预处理对大鼠急性肾缺血再灌注损伤的保护作用OACSTPCD

Objective:To observe the protective effect of pre-treatment with Yishen Huoxue Jiedu Formula on acute renal ischemia-reperfusion injury(IRI)in rats and explore its mechanism.Method:30 male SD rats were randomly divided into a blank group,a model group,and a Yishen Huoxue Jiedu Formula group(14.6 g·kg-1).The acute renal ischemia-reperfusion injury(IRI)rat model was established by ligating bilateral renal pedicle(45 minutes).Pre-treatment administration began 7 days before surgery,and the blank group and model group were given physiological saline by gavage once a day.After 24 hours of reperfusion,samples were taken and blood urea nitrogen(BUN),serum creatinine(SCr),and serum cystatin C(Cystatin C)levels were measured;HE staining was used to observe pathological changes in renal tissue and evaluate renal tubular injury score;Observation of ultrastructural changes in renal tubu-lar epithelial cells using transmission electron microscopy and calculation of lesion index;TUNEL method was used to detect apoptosis of renal tubular epithelial cells and calculate the apoptosis index.Result:(1)Compared with the blank group,the serum levels of BUN,SCr,and CysC in the model group rats were significantly increased(P<0.05);Compared with the model group,the serum BUN,SCr,and CysC levels of rats in the Yishen Huoxue Jiedu Formula group were significantly reduced(P<0.05).(2)HE staining:The renal tubular lumen of the model group rats was significantly dilated,with a large number of necrosis of renal tubular epithelial cells and ede-ma in the renal interstitium accompanied by a large number of lymphocyte infiltration;Compared with the blank group,the renal tubular injury score of the model group rats significantly increased(P<0.05);Compared with the model group,the pathological morphology of the kidney tissue in the Yishen Huoxue Jiedu Formula group was significantly improved,and the renal tubular injury score was signifi-cantly reduced(P<0.05).(3)Transmission electron microscopy:The renal tubules of the model group rats were severely damaged,with microvilli shedding and extracellular matrix accumulation,accompanied by a large number of mitochondrial vacuolar degeneration and endoplasmic reticulum expansion;Compared with the blank group,the ultrastructural lesion index of renal tubular epithelial cells in the model group rats was significantly increased(P<0.05);The Yishen Huoxue Jiedu Formula group of rats showed swelling and vac-uolar degeneration of a few mitochondria in the renal tubular epithelial cells,partial expansion of the endoplasmic reticulum,and a de-crease in the ultrastructural lesion index of renal tubular epithelial cells compared to the model group(P<0.05).(4)TUNEL:Com-pared with the blank group,the apoptosis index of renal tubular epithelial cells in the model group rats was significantly increased(P<0.05);Compared with the model group,the apoptosis index of renal tubular epithelial cells in the Yishen Huoxue Jiedu Formula group was significantly reduced(P<0.05).Conclusion:Pre-treatment with Yishen Huoxue Jiedu Formula has a protective effect on acute renal ischemia-reperfusion injury in rats,and its mechanism may be related to reducing damage to renal tubular epithelial cells and in-hibiting apoptosis of renal tubular epithelial cells.