针刺调控miR-665对帕金森病小鼠BNIP3介导的线粒体自噬的影响OACSTPCD

Objective:To explore the interaction of recombinant Bcl2/adenovirus E1B 19kDa interacting protein 3 in mice with Parkin-son's disease(PD)through acupuncture Effects of BNIP3 on mitochondrial autophagy.Methods:10 out of 55 BALB/c mice were ran-domly selected as sham operation group,and the other mice were injected subcutaneously with rotenone solution(1 mg·kg-1)once a day for 14 days.The 20 mice were randomly divided into model group and acupuncture group,with 10 mice in each group.Mice in sham operation group and model group were not interfered,and mice in acupuncture group were treated with acupuncture.The mRNA level of miR-665 in mouse striatum was detected by RT-PCR,and the helper T cells(TH)positive cells in mouse striatum were counted.The autonomic activity of mice was detected by open field test,and serum interleukin-1β(IL-1β),interleukin-8(IL-8),inter-leukin-6(IL-6)and tumor necrosis factor-α(TNF-α)levels were detected by ELISA.Western blot was used to detect the ex-pression levels of mito-LC 3Ⅱ,autophagy protein 5(Atg5),recombinant beclin 1(Beclin1)and BNIP3 in the striate of mice.Re-sults:Compared with sham operation group,the level of miR-665 mRNA in striatum of mice in model group and acupuncture group was increased(P<0.05).Compared with model group,the level of miR-665 mRNA in striatum of mice in acupuncture group was de-creased(P<0.05).Compared with sham operation group,the number of TH positive cells in brain tissue of mice in model group and acupuncture group decreased(P<0.05).Compared with model group,the number of TH positive cells in brain tissue of mice in acu-puncture group increased(P<0.05).Compared with sham operation group,the percentage of retarding time increased in model group and acupuncture group,and the movement time,average speed and total distance decreased(P<0.05).Compared with model group,the movement time,average speed,and total distance of mice in acupuncture group increased,and the percentage of stagnant time de-creased(P<0.05).Compared with sham operation group,serum levels of IL-1β,IL-6,TNF-α and IL-8 in model group and acu-puncture group were increased(P<0.05).Compared with model group,serum levels of IL-1β,IL-6,TNF-α and IL-8 in acu-puncture group were decreased(P<0.05).Compared with sham operation group,the protein expression levels of mito-LC3Ⅱ,Bec-lin1,Atg5 and BNIP3 in striatum of mice in model group and acupuncture group were increased(P<0.05).Compared with model group,the protein expression levels of mito-LC 3Ⅱ,Beclin1,Atg5 and BNIP3 in striatum of mice in acupuncture group were decreased(P<0.05).Conclusion:Acupuncture may affect BNIP3-mediated mitochondrial autophagy by downregulating the expression of miR-665,thereby improving the motor function and inflammatory response of PD mice.