目的 观察扁蒴藤素和顺铂对鼻咽癌细胞增殖和凋亡的影响,并探讨其作用机制.方法 CCK-8法检测不同浓度扁蒴藤素、顺铂处理24 h后HNE-1和CNE-2Z细胞的存活率,集落形成实验观察细胞集落形成能力,流式细胞术检测细胞凋亡和活性氧(ROS)水平,Western blotting检测蛋白表达.N-乙酰半胱氨酸(NAC)预处理后,检测细胞增殖、凋亡及通路的影响.结果 CCK-8结果显示,扁蒴藤素和顺铂均可抑制HNE-1和CNE-2Z细胞的存活率(P<0.05).与单用扁蒴藤素或顺铂相比,联合使用明显抑制细胞存活率和细胞集落形成能力(P<0.05),升高细胞凋亡率(P<0.01)和细胞内ROS水平(P<0.01),上调Bax、Cleaved caspase-3和Cleaved PARP的表达(P<0.05),下调Bcl-2、Mcl-1和PARP的表达(P<0.05),同时,下调细胞中p-PI3K和p-AKT的表达(P<0.05).而NAC可部分逆转扁蒴藤素联合顺铂对HNE-1和CNE-2Z细胞的增殖抑制(P<0.01)和凋亡诱导作用(P<0.05),部分恢复p-PI3K和p-AKT的下调作用(P<0.05).结论 扁蒴藤素能增强鼻咽癌细胞对顺铂的增殖抑制和凋亡诱导作用,其机制可能与ROS介导的PI3K/AKT信号通路失活有关.
Objective To explore the effect of pristimerin combined with cisplatin on proliferation and apoptosis of nasopharyngeal carcinoma cells.Methods CCK-8 assay was used to examine the survival rate of HNE-1 and CNE-2Z cells following treatment for 24 h with different concentrations of pristimerin,cisplatin or their combination.The changes in colony formation ability,apoptosis,and intracellular reactive oxygen species(ROS)levels of the treated cells were analyzed using colony formation assay and flow cytometry.Western blotting was performed to detect the changes in protein expressions in the cells.The effects of pre-treatment with NAC on proliferation,apoptosis,and PI3K/AKT signaling pathway were observed in pristimerin-and/or cisplatin-treated cells.Results Both pristimerin and cisplatin significantly lowered the survival rate of HNE-1 and CNE-2Z cells(P<0.05).Compared with pristimerin or cisplatin alone,their combination more strongly inhibited survival and colony formation ability of the cells,increased cell apoptosis rate and intracellular ROS levels,upregulated the protein expressions of Bax,cleaved caspase-3,and cleaved PARP,and downregulated the protein expressions of Bcl-2,Mcl-1,PARP and p-PI3K and p-AKT(P<0.05).NAC pretreatment significantly attenuated proliferation inhibition and apoptosis-promoting effects of pristimerin combined with cisplatin,and partially restored the downregulated protein expressions of p-PI3K and p-AKT in HNE-1 and CNE-2Z cells with the combined treatment(P<0.05).Conclusion Pristimerin can enhance cisplatin-induced proliferation inhibition and apoptosis in nasopharyngeal carcinoma cells,the mechanism of which may involve ROS-mediated deactivation of the PI3K/AKT signaling pathway.
王媛媛;陈腾;从小凡;李依然;陈蕊;张配;孙小锦;赵素容
蚌埠医科大学药学院//安徽省生化药物工程技术研究中心,安徽 蚌埠 233030
鼻咽癌;扁朔藤素;顺铂;活性氧;PI3K/AKT信号通路
nasopharyngeal carcinoma;pristimerin;cisplatin;reactive oxygen species;PI3K/AKT signaling pathway
《南方医科大学学报》 2024 (005)
904-912 / 9
国家自然科学基金(81603155);安徽省高校自然科学研究重点项目(KJ2021A0736,2022AH051534);蚌埠医学院自然科学重点项目(2021byzd018);安徽省大学生创新创业训练项目(S202210367013,S202210367109);蚌埠医学院研究生科研创新计划自然科学项目(Byycx22061) Supported by National Natural Science Foundation of China(81603155).
10.12122/j.issn.1673-4254.2024.05.12
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