荆鲜止痒涂剂治疗犬皮肤瘙痒症的作用机制探讨OA

In order to investigate the mechanism of action of Jingxian Zhiyang Tuji in the treatment of canis skin pruritus,network pharmacology method was adopted in this experiment.The active ingredients and their targets were screened by TCMSP,and all the targets were imported into Uniport database to form a drug-composition-target network.Genes related to canis skin diseases and pruritus were searched from GeneCards and OMIM databases.After intersection with the target of active drug ingredients,protein-protein interaction was mapped using STRING database and Cytoscape3.9 software.PPI network map was constructed and core targets were screened after visualization.Finally,GO and KEGG enrichment analysis was performed on common targets of drugs and diseases using DAVID database.The predicted active ingredients and core targets were verified by molecular docking technology.The results showed that 146 active ingredients were obtained,and the main components were quercetin,luteolin,apigenin,baicalein,β-sitosterol,cetoposide,sophorine,hypericin and isohesperetin.There were 55 targets associated with canine pruritus,among which the core targets were TNF,IL-6,VEGFA,etc.The core target is involved in the regulation of biological processes such as cytokine activity,extracellular space,apoptosis process,and positive regulation of RNA polymeraseⅡpromoter transcription,and acts on the regulation of MAPK signaling pathway,cytokine-cytokine receptor interaction signaling pathway,IL-17 signaling pathway and other signaling pathways in the anti-inflammatory,antibacterial,antioxidant and other processes to alleviate canis skin pruritus symptoms.The results of molecular docking showed that the key components of the drug had good binding with the core target.It showed that the treatment of canis pruritus with Jingxian Zhiyang Tuji paint involves multi-component,multi-target and multi-pathway mechanisms.