为探索不同诱导子对生物合成乌头碱产量的影响,本研究在黄曲霉Aspergillus flavus的液体发酵过程中分别加入金属诱导子(Co2+、Ag+和 Cu2+)和化学诱导子(茉莉酸甲酯、水杨酸和硝普钠),以及酵母提取物、种子提取物和共生细菌发酵液等生物诱导子进行诱导试验.试验结果表明,Ag+、Cu2+和Co2+都对乌头碱的生物合成有促进作用,其中Ag+浓度为50 μmol/L时,乌头碱产量与对照组相比提高了10倍.化学诱导子MJ和SA诱导效果显著,其中100 μmol/L MJ的加入能显著提高乌头碱的产量,产量为20.74 mg/L,为对照组的 11 倍.而酵母提取物、种子提取物和共生细菌发酵液对乌头碱的产量诱导效果均不显著.本论文探究了不同诱导子对黄曲霉生物合成乌头碱的诱导作用,进而明确不同诱导子及不同诱导浓度对乌头碱生物合成产量的影响.
In order to explore the effect of different inducers on the yield of biosynthesis aconitine,the present study was carried out in the liquid fermentation of Aspergillus flavus by adding metal inducers(Co2+,Ag+and Cu2+)and chemical inducers(Methyl Jasmonate,MJ,Salicylic Acid,SA and Sodium nitroprusside,SND),as well as YE(Yeast extract),SE(Seed extract)and Bac(Symbiotic bacterial fermentation broth).The results showed that Ag+,Cu2+and Co2+all could promote the biosynthesis of aconitine,in which Ag+at 50 μmol/L increased the aconitine yield by 10-fold compared with the control.Induction effects of the chemical inducers MJ and SA were significant,in which the addition of 100 μmol/L MJ significantly increased the production of aconitine with a yield of 20.74 mg/L,which was 11-fold higher than that of the control,while YE,SE and Bac did not have any significant effect on the biosynthesis of aconitine by Aspergillus flavus.
王凯;袁希元;张明月;田硕;何璐
沈阳农业大学植物保护学院,沈阳 110866
植物保护学
诱导子;黄曲霉菌;乌头碱;生物合成
inducer;Aspergillus flavus;aconitine;biosynthesis
《中国生物防治学报》 2024 (002)
469-476 / 8
辽宁省民生科技计划(2021JH2/10200016)
10.16409/j.cnki.2095-039x.2024.02.015
评论