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磷脂与大豆异黄酮对黄羽肉鸡生长性能、肉品质及抗氧化功能的影响OA北大核心CSTPCD

Effects of Phospholipids and Soybean Isoflavones on Growth Performance,Meat Quality,and Antioxidant Function of Yellow-feathered Broilers

中文摘要英文摘要

[目的]本试验旨在研究饲粮中添加磷脂与大豆异黄酮对黄羽肉鸡生长性能、肉品质和肌肉中脂肪酸组成的影响,以期通过营养调控提高肉鸡生长性能和肉品质,为促进黄羽肉鸡提质增效提供理论依据和技术支撑.[方法]试验采用2(添加或不添加大豆异黄酮)×3(不添加磷脂、添加大豆磷脂或溶血磷脂)双因子设计.选用900只健康、体重一致的48日龄中速型黄羽肉鸡母鸡,随机分为6个处理组,每组6个重复,每个重复25只鸡.第1组饲粮添加3.00%菜籽油(对照组);第2组饲粮添加1.00%大豆磷脂,等量替代第1组饲粮中的菜籽油;第3组在第1组基础上添加500 mg/kg溶血磷脂;第4-6组分别在第1~3组基础上添加300 mg/kg大豆异黄酮.试验期32 d.于80日龄以重复为单位对肉鸡进行空腹称重并统计采食量,计算生长性能;每重复选取1只接近平均体重的肉鸡采血和屠宰,测定屠宰性能和血浆生化指标;取胸肌评价肉品质,并测定丙二醛(MDA)、挥发性盐基氮(TVBN)含量和脂肪酸组成.[结果]与对照组相比,在48~80日龄中速型黄羽肉鸡母鸡饲粮中添加磷脂与大豆异黄酮均可显著降低肉鸡死亡率(P<0.05),并且存在显著交互作用(P<0.05);饲粮中添加磷脂或大豆异黄酮均可显著降低血浆中的氧化型谷胱甘肽(GSSG)和MDA含量(P<0.05),均可显著增加血浆中还原型谷胱甘肽(GSH)/GSSG比值(P<0.05);饲粮中添加溶血磷脂或大豆异黄酮均能显著降低肉鸡肌肉蒸煮损失(P<0.05);饲粮中添加磷脂可显著增加宰后45 min胸肌肉色红度值(a*)和单不饱和脂肪酸含量(P<0.05),并显著降低n-6多不饱和脂肪酸(n-6 PUFA)含量(P<0.05);饲粮中添加大豆磷脂可以显著降低胸肌中MDA和挥发性盐基氮含量(P<0.05);饲粮中添加大豆异黄酮可以显著增加血浆中高密度脂蛋白胆固醇和GSH含量以及胸肌中PUFA、n-3 PUFA、亚油酸和亚麻酸含量(P<0.05),并可显著降低血浆中甘油三酯含量和胸肌中n-6 PUFA/n-3 PUFA值(P<0.05).[结论]在48~80日龄中速型黄羽肉鸡母鸡饲粮中单独添加磷脂与大豆异黄酮均可提高机体的抗氧化能力,改善胸肌中脂肪酸组成,提高肉品质.

[Objective]This experiment was conducted to investigate the effects of phospholipid(PL)and soybean isoflavone(ISO)supplementation on growth performance,meat quality and fatty acid composition in the breast muscle of Yellow-feathered broilers,in order to improve growth performance and meat quality of broilers through nutritional regulation,and to provide theoretical basis and technical support for improving quality and efficiency of Yellow-feathered broilers.[Method]The experiment was 2(with or without ISO in diet)X 3(control,soybean phospholipid(SL)or lysophospholipid(LPL)in diet)factorial design.A total of 900 48-day-old female medium-growing Yellow-feathered broilers were randomly divided into 6 groups with 6 replicates per group and 25 broilers per replicate.The broilers in group 1 were fed basal diet supplemented with 3.00%rapeseed oil(control group).On the basis of control group,1.00%SL were used to replace rapeseed oil in the diet in group 2(SL group).On the basis of group 1,500 mg/kg LPL products was supplemented in group 3,and in groups 4-6,300 mg/kg ISO products were supplemented on the basis of groups 1-3,respectively.The experiment lasted for 32 days.At 80 days of age,the broilers were weighed with fasting and feed intake was analysed to calculate the growth performance.One broiler with average body weight was selected for blood collection and slaughter,and the slaughter performance and plasma biochemical indexes were determined.The breast muscle was taken to evaluate the meat quality,malondialdehyde(MDA)content,volatile basic nitrogen(TVBN)content and fatty acid composition.[Result]Compared to control group,supplementation of PL and ISO in the diets of female medium-growing Yellow-feathered broilers aged 48 to 80 days could significantly reduce the mortality of broilers(P<0.05),and there was an interaction between them.Dietary PL or ISO supplementation significantly decreased the contents of GSSG and MDA in plasma(P<0.05),significantly increased the ratio of GSH/GSSG in plasma(P<0.05).Dietary LPL or ISO supplementation significantly decreased cooking loss of breast muscle(P<0.05).Dietary PL supplementation significantly increased a45*min and the contents of monounsaturated fatty acid(P<0.05),and significantly decreased the content of n-6 polyunsaturated fatty acid(n-6 PUFA)in breast muscle(P<0.05).Dietary SL supplementation significantly decreased MDA and TVBN contents in breast muscle(P<0.05).Dietary ISO supplementation significantly increased plasma HDL-C and GSH content and the contents of PUFA,n-3 PUFA,linoleic acid and linolenic acid in breast muscle(P<0.05),and significantly decreased plasma TG content and n-6 PUFA/n-3 PUFA values in breast muscle(P<0.05).[Conclusion]The supplementation of PL or ISO in the diet of female medium-growing Yellow-feathered broilers of 48 to 80 days of age could increase the antioxidant capacity,optimize the fatty acid composition in breast muscle,and improve the meat quality.

叶金玲;范秋丽;王一冰;林厦菁;阮栋;罗琦丽;苟钟勇;蒋守群

广东省农业科学院动物科学研究所,猪禽种业全国重点实验室,农业农村部华南动物营养与饲料重点实验室,广东省畜禽育种与营养研究重点实验室,广州 510640

畜牧业

中速型黄羽肉鸡;磷脂;大豆异黄酮;生长性能;肉品质;抗氧化性能;脂肪酸组成

medium-growing Yellow-feathered broilers;phospholipids;soybean isoflavones;growth performance;meat quality;antioxidant capacity;fatty acid composition

《中国畜牧兽医》 2024 (005)

1857-1867 / 11

现代农业产业技术体系国家肉鸡产业技术体系(CARS-41);国家重点研发计划项目(2021YFD1300404);广东省重点领域研发计划项目(2020B0202090004);广东省自然科学基金项目(2021A1515012412)

10.16431/j.cnki.1671-7236.2024.05.007

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