[目的]探讨疆南绒山羊皮肤毛囊的生长发育机制,加深对次级毛囊发育相关候选基因遗传调控和功能的认识,为指导疆南绒山羊后期培育,改善其绒产量提供参考.[方法]利用高通量测序技术对疆南绒山羊次级毛囊生长期、退行期和休止期皮肤组织中环状RNA(circRNA)进行分析,筛选出不同比较组的差异表达circRNA(DE circRNA),对其宿主基因进行GO功能、KEGG通路及蛋白互作(PPI)分析,并联合前期转录组数据构建竞争性内源RNA(ceRNA)网络.[结果]在9个疆南绒山羊皮肤组织中共鉴定到2 482个circRNAs.其中包含138个DE circRNAs,退行期和生长期、退行期和休止期、休止期和生长期比较组中分别存在54、74、65个DE circRNAs.GO功能和KEGG通路分析揭示,DE circRNA宿主基因主要富集在细胞黏附、整合素介导信号通路、细胞整合素复合物等功能通路,以及一些与毛囊生理过程有关的信号通路,如MAPK、TGF-beta、Hedgehog等.结合PPI网络推测14个circRNAs可能参与调控疆南绒山羊皮肤次级毛囊生长发育.通过6个DE circRNAs、20个DE miRNAs、3个DE mRNAs和2个DE lncRNAs构建了疆南绒山羊ceRNA网络.[结论]本研究通过转录组测序筛选出与疆南绒山羊次级毛囊周期发育相关的关键circRNAs,包括novel-gene5238-2、novel-gene7855-1、novel-gene4455-1等.研究结果为circRNA在绒山羊次级毛囊发育中的生物学功能和调控特性提供理论依据,也为疆南绒山羊育种提供了新的方向.
[Objective]This study was aimed to investigate the mechanism of growth and development in skin hair follicles of Jiangnan cashmere goats,enhance comprehension of the genetic regulation and function of candidate genes related to secondary hair follicle development,and provide guidance for future breeding of Jiangnan cashmere goats to improve their fleece production.[Method]The high-throughput sequencing technology was used to carry out transcriptomic analysis of circular RNA(circRNA)in the skin tissues of secondary hair follicles in Jiangnan cashmere goats during anagen,catagen and telogen phases.The differentially expressed circRNA(DE circRNA)in different comparison groups was selected,and the host genes were analyzed by GO function,KEGG pathway and protein-protein interaction(PPI).The competitive endogenous RNA(ceRNA)network was constructed by combining the preliminary transcriptome data.[Result]A total of 2 482 circRNAs were identified in 9 skin tissues of Jiangnan cashmere goats,of which 138 were DE circRNAs.In the comparison group of phases,there were 54,74 and 65 DE circRNAs were found in the comparison group of catagen and anagen,catagen and telogen,telogen and anagen phases,respectively.GO function and KEGG pathway analysis revealed that DE circRNA host genes were mainly concentrated in functional pathways such as cell adhesion,integrin-mediated signaling pathway,cell integrin complex,and some signaling pathways related to physiological processes of hair follicles such as MAPK,TGF-beta and Hedgehog.Combining with the PPI network,it was speculated that 14 circRNAs might participate in the regulation of growth and development of secondary hair follicles in Jiangnan cashmere goats.In addition,6 DE circRNAs,20 DE miRNAs,3 DE mRNAs and 2 DE lncRNAs were constructed in the ceRNA network of Jiangnan cashmere goats.[Conclusion]In this study,transcriptome sequencing was used to screen essential circRNAs related to secondary hair follicle cycle development in Jiangnan cashmere goats,including novel-gene5238-2,novel-gene7855-1,novel-gene4455-1,etc.The results provided theoretical foundation for the biological function and regulatory property of circRNA in the development of secondary hair follicles in cashmere goats,and provided a new prospect for breeding of Jiangnan cashmere goats.
陆庆伟;唐森;李彬;秦崇凯;刘文娜;蒲雪;王亚倩;吴翠玲;付雪峰
新疆农业大学动物科学学院,乌鲁木齐 830052||新疆畜牧科学院畜牧研究所,新疆绒毛用羊遗传育种与繁殖实验室(XJYS1105),乌鲁木齐 830000新疆畜牧科学院生物技术研究所,农业农村部草食家畜遗传育种与繁殖重点实验室,乌鲁木齐 830000阿克苏地区畜牧技术推广中心,阿克苏 843000新疆畜牧科学院畜牧研究所,新疆绒毛用羊遗传育种与繁殖实验室(XJYS1105),乌鲁木齐 830000||新疆师范大学生命科学学院,新疆特殊环境物种保护与调控生物学实验室/新疆特殊环境物种多样性应用与调控重点实验室,乌鲁木齐 830017新疆师范大学生命科学学院,新疆特殊环境物种保护与调控生物学实验室/新疆特殊环境物种多样性应用与调控重点实验室,乌鲁木齐 830017新疆畜牧科学院畜牧研究所,新疆绒毛用羊遗传育种与繁殖实验室(XJYS1105),乌鲁木齐 830000
畜牧业
疆南绒山羊;转录组测序;环状RNA(circRNA);次级毛囊周期
Jiangnan cashmere goats;transcriptome sequencing;circular RNA(circRNA);secondary hair follicle cycle
《中国畜牧兽医》 2024 (005)
1793-1806 / 14
国家重点研发计划(2021YFD1200902);国家自然科学基金(32360814);新疆维吾尔自治区自然科学基金(2022D01B107);新疆维吾尔自治区"天池英才";新疆自治区创新环境建设专项(2021D04008)
10.16431/j.cnki.1671-7236.2024.05.001
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