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6份毛木耳种质氨基酸组成及其营养评价OA北大核心CSTPCD

Amino acid composition and nutritional evaluation of six Auricularia cornea Ehrenb.germplasms

中文摘要英文摘要

[目的]探究不同毛木耳种质的氨基酸组成及营养差异,为合理开发利用毛木耳资源提供科学参考.[方法]测定6份毛木耳种质(SH、QB、CB、FS、HY和CF)的氨基酸和常规营养成分,基于主成分分析、聚类分析,利用氨基酸比值(RAA)、氨基酸比值系数分(SRC)等指标综合评价营养差异.[结果]6份毛木耳种质均含17种氨基酸,氨基酸总量4.139~6.314 g/100 g,以QB的氨基酸总量最高,且显著高于其他5份种质(P<0.05,下同);天门冬氨酸(Asp)和谷氨酸(Glu)含量相对较高,半胱氨酸(Cys)、蛋氨酸(Met)、酪氨酸(Tyr)和异亮氨酸(Ile)含量相对较低;第一限制性氨基酸为Met+Cys,第二限制性氨基酸为赖氨酸(Lys),呈味氨基酸以鲜味氨基酸为主,必需氨基酸与总氨基酸比值均高于联合国粮食及农业组织/世界卫生组织(FAO/WHO)模式;SRC为63.999~75.217,以FS和CB相对较高.不同毛木耳种质营养差异明显,必需氨基酸含量1.515~2.227 g/100 g,QB和HY显著高于其他种质;蛋白质含量5.508~7.707 g/100 g,以HY最高;粗多糖含量3.827~10.140 g/100 g,钙含量1.790×103~4.867×103 mg/kg,铁含量17.007~180.667 mg/kg,灰分含量1.967~5.400 g/100 g,均以FS最高;粗脂肪含量0.100~0.500 g/100 g,锌含量6.333~10.800 mg/kg,硒含量0.015~0.084 mg/kg,均以CB最高,且显著高于其他种质.主成分分析结果显示,6份种质氨基酸综合得分由高到低排序为QB>HY>FS>CF>CB>SH.聚类分析将6份种质分成三大类群,SH、CB和CF聚为类群Ⅰ,综合品质较差;FS单独聚为类群Ⅱ,综合品质居中;QB和HY聚为类群Ⅲ,综合品质最佳.[结论]6份毛木耳种质氨基酸种类齐全,必需氨基酸占比较高,营养均衡性好;不同毛木耳种质的氨基酸组成及营养品质差异明显,以QB和HY的综合营养品质最佳.

[Objective]The purpose of the study was to investigate the amino acid composition and nutritional diffe-rences among various Auricularia cornea Ehrenb.germplasms,and to provide a scientific reference for the rational deve-lopment and utilization of A.cornea resources.[Method]Amino acids and conventional nutritional components of six A.cornea germplasms(SH,QB,CB,FS,HY and CF)were determined.Comprehensive evaluation of nutritional diffe-rences was conducted based on principal component analysis,cluster analysis,and indicators such as amino acid ratio(RAA)and amino acid ratio coefficient score(SRC).[Result]All six germplasms of A.cornea contained seventeen types of amino acids,and the total amino acid content ranged from 4.139 g/100 g to 6.314 g/100 g.The total amino acid content of QB was the highest,and was significantly higher than that of the other five germplasm(P<0.05,the same below).The contents of aspartate(Asp)and glutamic acid(Glu)were relatively high,and the contents of cysteine(Cys),methio-nine(Met),tyrosine(Tyr)and isoleucine(Ile)were relatively low.The first limiting amino acid was Met+Cys and the second limiting amino acid was lysine(Lys).The flavor amino acids were mainly umami amino acids,and the ratios of essential amino acids to total amino acids were higher than that of the Food and Agriculture Organization of the United Na-tions/World Health Organization(FAO/WHO)model.SRC ranged from 63.999 to 75.217,with FS and CB being rela-tively high.Obvious nutritional differences were observed among different A.cornea germplasms.Essential amino acid content ranged from 1.515 to 2.227 g/100 g,with QB and HY being significantly higher than other germplasms.Protein content ranged from 5.508 to 7.707 g/100 g,with HY being the highest.Crude polysaccharide content ranged from 3.827 to 10.140 g/100 g,calcium content ranged from 1.790×103 to 4.867×103 mg/kg,ferrum content ranged from 17.007 to 180.667 mg/kg,and ash content ranged from 1.967 to 5.400 g/100 g,all with FS being the highest.Crude fat content ranged from 0.100 to 0.500 g/100 g,zinc content ranged from 6.333 to 10.800 mg/kg,and selenium content ranged from 0.015 to 0.084 mg/kg,with CB being the highest and significantly higher than the other germplasms.Principal component analysis result showed that the comprehensive amino acid scores of the six germplasms were ranked QB>HY>FS>CF>CB>SH.Cluster analysis grouped the six germplasms into three categories.SH,CB and CF were clustered into groupⅠwith relatively poor overall quality.FS was clustered individually into groupⅡwith moderate overall quality.QB and HY clustered into group Ⅲ with the best overall quality.[Conclusion]The tested six A.cornea germplasm exhibit a complete range of amino acids,a higher proportion of essential amino acids and good nutritional balance.Obvious differences are observed in amino acid composition and nutritional quality among different A.cornea germplasms,with QB and HY dis-playing the best overall nutritional quality.

叶雷;张波;杨学圳;谭伟;李小林

四川省食用菌研究所,四川成都 610066||四川农业大学资源学院,四川成都 611130四川省食用菌研究所,四川成都 610066

园艺学与植物营养学

毛木耳;种质;氨基酸;主成分分析;营养评价

Auricularia cornea Ehrenb.;germplasm;amino acid;principal component analysis;nutritional evalua-tion

《南方农业学报》 2024 (002)

566-577 / 12

国家现代农业产业技术体系建设专项(CARS-20);四川省科技计划项目(2021YFYZ0026);四川食用菌创新团队项目(SCCXTD-2024-07);德阳市科技计划项目(重点研发)(2022NZ019) China Agriculture Research System Construction Project(CARS-20);Sichuan Science and Technology Plan Project(2021YFYZ0026);Sichuan Edible Mushroom Innovation Team Project(SCCXTD-2024-07);Science and Technology Plan Project of Deyang City(Key Research and Development)(2022NZ019)

10.3969/j.issn.2095-1191.2024.02.026

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