活性内生细菌对PEG-6000胁迫下小麦生理指标的影响OA

The experiment used Jimai 22 wheat as the experimental material and simulated drought stress(20%PEG-6000)under hydroponic conditions.During the stress cultivation process,four strains of fig active endophytic bacteria(Bacillus subtilis strain 3251,Bacillus velezensis strain 3252,Bacillus WHG5181,Bacillus WHG5182)were introduced,and the effects of four active endophytic bacteria on the physiological characteristics of wheat seedlings under drought stress were analyzed by measuring the plant height,root activity and proline content of wheat.The results showed that four strains of fig active endophytic bacteria had a certain effect on alleviating drought stress of wheat seedlings.The plant height of wheat treated with 20%PEG-6000+active endophytic bacteria was higher than that of the blank control and 20%PEG-6000 treatment.On the 7th-21th day of stress cultivation,the root activity of wheat treated with 20%PEG-6000+Bacillus WHG5182 was higher than that of wheat treated with the 20%PEG-6000,and the increase of root TTC reduction intensity was 70.78%,4.80%and 12.67%,respectively.On the 14th day of stress culture,the proline content in the leaves of wheat treated with 20%PEG-6000+Bacillus WHG5182 was 91.96%higher than that of wheat treated with the 20%PEG-6000.It indicates that the active endophytic bacterium Bacillus WHG5182 can effectively alleviate the impact of drought stress on wheat growth in a certain period of time.