目的 分析桔梗皂苷D对大鼠力竭运动性肾损伤的保护作用.方法 用跑台训练建立力竭运动性肾损伤模型.将50只SD大鼠分为对照组(灌胃等量0.9％NaCl)、模型组(力竭运动模型)和低、中、高剂量实验组(分别灌胃25、50、100 mg·kg-1桔梗皂苷D).用试剂盒检测肾功能[尿素氮(BUN)、血肌酸酐(Scr)]、肾损伤标志物[中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、肾损伤分子-1(KIM-1)]水平;用蛋白质印迹法检测核苷酸寡聚化结构域样受体家族3(NLRP3)、凋亡相关斑点样蛋白(ASC)、胱天蛋白酶-1(Caspase-1)蛋白表达.结果 对照组、模型组和低、中、高剂量实验组BUN水平分别为(5.52±0.49)、(9.98±0.85)、(8.30±0.65)、(7.12±0.47)和(5.98±0.63)mmol·L-1,Scr水平分别为(1.69±0.24)、(5.69±0.67)、(4.71±0.47)、(3.55±0.25)和(2.67±0.24)ng·mL-1,NGAL 水平分别为(207.58±28.56)、(613.42±63.87)、(521.52±55.35)、(419.89±40.85)和(278.13±26.48)pg·mL-1,KIM-1 水平分别为(12.11±1.52)、(80.42±9.56)、(69.03±7.58)、(47.32±6.67)和(24.67±3.60)ng·L-1,NLRP3 蛋白表达水平分别为0.43±0.06、1.35±0.11、1.03±0.10、0.78±0.09 和 0.53±0.06,ASC 蛋白表达水平分别为 0.25±0.03、0.74±0.06、0.60±0.06、0.44±0.05 和 0.31±0.04,Caspase-1 蛋白表达水平分别为 0.31±0.04、0.83±0.07、0.69±0.06、0.53±0.05 和 0.42±0.0 4.模型组上述指标与对照组比较,在统计学上差异均有统计学意义(均P＜0.01);低、中、高剂量实验组上述指标与模型组比较,在统计学上差异均有统计学意义(均P＜0.01).结论 桔梗皂苷D可通过抑制NLRP3炎性小体激活进而减轻大鼠力竭运动性肾损伤.
Objective To analyze the protective effect of platycodon platycodon D on renal injury induced by exercise exhaustion in rats.Methods The model of kidney injury induced by exhaustive exercise was established by treadmill training.Fifty SD rats were divided into control group(0.9％NaCl),model group(exhaustive exercise model),experimental-L,-M,-H groups(25,50,100 mg·kg-1 platycodon D).Renal function[blood urea nitrogen(BUN),serum creatinine(Scr)],renal injury markers[neutrophil gelatinase associated lipocalin(NGAL),kidney injury molecule-1(KIM-1)]were detected by the kit;nucleotide oligomeric domain-like receptor family 3(NLRP3),apoptosis-associated speck-like protein containing a card(ASC),cysteine aspartic acid specific protease-1(Caspase-1)protein expressions were detected by Western blot.Results BUN levels in control group,model group,experimental-L,-M,-H groups were(5.52±0.49),(9.98±0.85),(8.30±0.65),(7.12±0.47)and(5.98±0.63)mmol·L-1;Scr were(1.69±0.24),(5.69±0.67),(4.71±0.47),(3.55±0.25)and(2.67±0.24)ng·mL-1;NGAL were(207.58±28.56),(613.42±63.87),(521.52±55.35),(419.89±40.85)and(278.13±26.48)pg·mL-1;KIM-1 were(12.11±1.52),(80.42±9.56),(69.03±7.58),(47.32±6.67)and(24.67±3.60)ng·L-1;NLRP3 protein expression were 0.43±0.06,1.35±0.11,1.03±0.10,0.78±0.09 and 0.53±0.06;ASC protein expression were 0.25±0.03,0.74±0.06,0.60±0.06,0.44±0.05 and 0.31±0.04;Caspase-1 protein expression were 0.31±0.04,0.83±0.07,0.69±0.06,0.53±0.05 and 0.42±0.04.Compared with the control group,the above indexes in the model group were statistically significant(all P＜0.01);compared with the model group,the above indexes of the experimental-L,-M,-H groups were statistically significant(all P＜0.01).Conclusion Platycodon D can inhibit the activation of NLRP3 inflammasome to alleviate the renal injury induced by exhaustive exercise in rats.
苏龙;陶瑞松
合肥师范学院体育科学学院,安徽合肥 230601合肥师范学院生命与食品工程学院,安徽合肥 230601
中医学
桔梗皂苷D;力竭运动;肾功能;炎性反应;核苷酸寡聚化结构域样受体家族3炎性小体
platycodon D;exhaustive exercise;kidney function;inflammatory response;nucleotide oligomeric domain-like receptor family 3 inflammasome
《中国临床药理学杂志》 2024 (008)
1194-1197 / 4
安徽省高校自然科学基金资助项目(KJ2020A0144)
10.13699/j.cnki.1001-6821.2024.08.022
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