目的 探讨中药"济川煎"对于慢传输型便秘(STC)大鼠排便情况的影响.方法 将36只SD大鼠随机分为模型组、实验组和对照组,除对照组外,其余2组均用2％大黄酸混悬液灌胃法建立STC大鼠模型,实验组用170％济川煎浓缩液10 mL·kg-1灌胃,对照组和模型组用0.9％NaCl灌胃,每天灌胃1次,连续30 d.通过粪便称质量、记录首次粪便排出时间计算粪便干湿质量比及排便时间,用试剂盒检测血浆中P物质(SP)及血管活性肽(VIP)含量,用实时荧光定量聚合酶链反应(qRT-PCR)技术检测肠道菌群变化.结果 对照组、模型组和实验组粪便干湿质量比分别为(44.86±4.40)％、(53.44±3.52)％和(44.28±4.33)％,排便时间分别为(340.17±34.91)、(424.67±37.26)和(377.36±38.87)min,血浆中 SP 分别为(79.86±7.56)、(60.30±2.41)和(78.84±5.83)pg·mL-1,血浆中 VIP 分别为(30.56±2.87)、(23.38±6.62)和(28.39±2.37)pg·mL-1,双歧杆菌分别为 7.77±0.04、5.19±0.09 和 7.45±0.03;乳酸杆菌数分别为6.40±0.07、4.10±0.05和6.36±0.08.模型组以上指标与对照组比较,实验组以上指标与模型组比较,在统计学上差异均有统计学意义(P＜0.05,P＜0.01).结论 中药"济川煎"能够改善慢传输型便秘大鼠的排便情况.
Objective To investigate the effect of Jichuan decoction on defecation in rats with slow transit constipation(STC).Methods A total of 36 SD rats were divided into model group,experimental group and control group.Except the control group,STC rat models were established in the other two groups by gavage of 2％rhein acid suspension.The experimental group was given 170％Jichuan decoction of 10 mL·kg-1 by gavage,while the control group and model group were given with 0.9％NaCl by gavage,once a day for 30 days.Fecal dry-wet weight ratio and colon transport time were observed by fecal weighing and recording of first fecal discharge time;substance P(SP)and vasoactive peptide(VIP)contents in plasma were detected by kit;changes in intestinal flora were detected by real-time fluorescent quantitative polymerase chain reaction(qRT-PCR).Results The dry-wet ratio of feces in control group,model group and experimental group were(44.86±4.40)％,(53.44±3.52)％and(44.28±4.33)％,respectively;the colon transport time were(340.17±34.91),(424.67±37.26)and(377.36±38.87)min,respectively;plasma SP were(79.86±7.56),(60.30±2.41)and(78.84±5.83)pg·mL-1,respectively;plasma VIP were(30.56±2.87),(23.38±6.62)and(28.39±2.37)pg·mL-1,respectively;the numbers of Bifidobacterium were 7.77±0.04,5.19±0.09 and 7.45±0.03,respectively;the numbers of Lactobacillus were 6.40±0.07,4.10±0.05 and 6.36±0.08,respectively.There were statistically significant differences in the above indexes between model group and control group,and between experimental group and model group(P＜0.01,P＜0.05).Conclusion Jichuan decoction can improve the defecation of rats with slow transit constipation.
裴贵兵;张波;陈志成;姜华
东南大学附属中大医院肛肠外科,江苏南京 210009东南大学附属中大医院泌尿外科,江苏南京 210009
中医学
济川煎;慢传输型便秘;血管活性肠肽;P物质;肠道菌群
Jichuan decoction;slow transit constipation;vasoactive intestinal peptide;substances P;intestinal flora
《中国临床药理学杂志》 2024 (008)
1189-1193 / 5
江苏省中医药局科技基金资助项目(YB2017099);江苏省老年健康科研基金资助项目(LX2021016)
10.13699/j.cnki.1001-6821.2024.08.021
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