目的 探讨俞氏清热活血方灌肠对溃疡性结肠炎(ulcerative colitis,UC)大鼠结肠黏膜的影响.方法 30 只Wistar大鼠分为空白对照组、模型组、美沙拉嗪组和中药高、中、低剂量组,每组各 5 只;使用三硝基苯磺酸造模成功后,分别予蒸馏水、美沙拉嗪混悬液与高、中、低浓度俞氏清热活血方灌肠 14d,每日记录各组大鼠的一般情况及体质量变化,14d后比较各组大鼠的结肠黏膜损伤指数(colon macroscopic damage index,CMDI)及镜下黏膜损伤评分.结果 与空白对照组比较,模型组大鼠体质量显著下降(P<0.05).空白对照组大鼠的饮水、摄食、活动正常,毛色润泽,大便正常;模型组和各给药组大鼠均可见肛门污秽、有便血残留、食欲下降、毛发凌乱、光泽暗淡等情况,灌肠治疗后上述状况均逐渐好转.与空白对照组比较,模型组大鼠的 CMDI 及黏膜损伤评分显著升高(P<0.05);与模型组比较,美沙拉嗪组和中药高、中、低剂量组大鼠的CMDI及黏膜损伤评分均显著下降(P<0.05).结论 俞氏清热活血方可有效改善UC模型大鼠的腹泻、黏液脓血便等临床症状,减少结肠黏膜损伤,促进黏膜修复,对治疗UC具有较好的疗效.
Objective To investigate the effect of Yu's Qingre Huoxue prescription enema on colonic mucosa in rats with ulcerative colitis(UC).Methods A total of 30 Wistar rats were randomly divided into blank control group,model group,mesalazine group,Chinese medicine high-dose,medium-dose and low-dose groups(5 rats in each group).After successful modeling with trinitrobenzene sulfonic acid,the rats were given 14 days enema with distilled water,mesalazine suspension,and high,medium and low concentration Yu's Qingre Huoxue prescription.The general condition and weight changes of rats were recorded daily,and colon macroscopic damage index(CMDI)and mucosal injury score under microscope of rats in each group were compared.Results Compared with blank control group,the weight of rats in model group decreased significantly(P<0.05).The blank control group had normal water and food intake and activity,glossy fur and normal stool.The rats in model group and each administration group were found to have anal fouling,residual blood in stool,decreased appetite,messy fur,dim luster and other conditions,and the above conditions were gradually improved after enema treatment.Compared with blank control group,the CMDI and mucosal injury scores of model group were significantly increased(P<0.05).Compared with model group,CMDI and mucosal injury scores of mesalazine group and Chinese medicine high-dose,medium-dose and low-dose groups were significantly decreased(P<0.05).Conclusion Yu's Qingre Huoxue prescription can effectively improve the clinical symptoms of diarrhea,mucous,pus and blood stools in UC model rats,reduce the damage of colonic mucosa,promote mucosal repair,and have a good effect on the treatment of UC in rats.
杨一天;陈雯婧;叶蔚
浙江中医药大学附属杭州市中医院脾胃病科,浙江杭州 310007浙江中医药大学第三临床医学院,浙江杭州3100531
临床医学
俞氏清热活血方;溃疡性结肠炎;结肠黏膜损伤指数;结肠黏膜损伤评分
Yu's Qingre Huoxue prescription;Ulcerative colitis;Colon macroscopic damage index;Colonic mucosal injury score
《中国现代医生》 2024 (010)
56-59 / 4
浙江省杭州市医药卫生科技计划项目(0020190373)
10.3969/j.issn.1673-9701.2024.10.014
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