目的 探讨二乙酰己二胺(diacetyl hexamethylene diamine,CAHB)诱导成人急性淋巴细胞白血病Jurkat细胞凋亡的机制,为CAHB应用于临床提供理论依据.方法 采用流式细胞仪分析CAHB处理后Jurkat细胞AnnexinⅤ+/PI-细胞率,观察应用胱天蛋白酶(caspase)-9 抑制剂Z-LEHD-FMK处理后AnnexinⅤ+/PI-细胞率的变化;蛋白质印迹法观察凋亡相关蛋白caspase-8、caspase-9、caspase-3 的表达变化.结果 CAHB诱导后Jurkat细胞体积缩小、胞膜皱缩、染色质浓染、核固缩或碎裂等,可见典型凋亡小体.CAHB通过激活caspase-9、caspase-3 诱导Jurkat细胞发生凋亡,其作用呈明显的量效关系和时间依赖性,caspase-9 抑制剂可在一定程度上抑制CAHB的凋亡诱导作用.结论 CAHB诱导Jurkat细胞凋亡与激活caspase-9 及caspase-3有关.
Objective To explore the mechanism of apoptosis induced by diacetyl hexamethylene diamine(CAHB)in adult acute lymphoblastic leukemia Jurkat cells,and to provide theoretical basis for the clinical application of CAHB.Methods Annexin V+/PI-cell rate of Jurkat cells after CAHB induction was analyzed by flow cytometry.The Annexin V+/PI-cell rate was observed after treatment with caspase-9 inhibitor Z-LEHD-FMK.The expressions of apoptosis-related proteins caspase-8,caspase-9 and caspase-3 were observed by Western blotting.Results After CAHB induction,Jurkat cells were reduced in size,cell membrane crinkling,chromatin thickening,nuclear pyknosis or fragmentation,etc.Typical apoptotic bodies could be seen.CAHB induced Jurkat cell apoptosis by activating caspase-9 and caspase-3 in a dose-effect and time-dependent manner.Caspase-9 inhibitors could inhibit apoptosis induction of CAHB to a certain extent.Conclusion CAHB induced Jurkat cell apoptosis was related to caspase-9 and caspase-3 activation.
张佳;洪叶;陈葆国;罗文达
浙江省台州医院血液内科,浙江临海 3170001
临床医学
二乙酰己二胺;急性淋巴细胞白血病;Jurkat细胞;凋亡
Diacetyl hexamethylene diamine;Acute lymphoblastic leukemia;Jurkat cell;Apoptosis
《中国现代医生》 2024 (010)
39-42 / 4
10.3969/j.issn.1673-9701.2024.10.010
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