目的 探讨 MEX3A 与胃癌和肠上皮化生(以下简称肠化生)分化特性的相关性及其联合尾型同源盒转录因子 2(caudal-related homeobox transcription factor 2,CDX2)、黏蛋白 2(mucin 2,MUC2)和黏蛋白 5AC(mucin 5AC,MUC5AC)判断可癌变肠化生的作用.方法 选取 2010 年 1 月至 2014 年 12 月沈阳医学院附属中心医院、沈阳医学院附属第二医院外科手术切除的胃癌及癌旁石蜡包埋组织样本 410 例,根据病理诊断将其分为对照组(轻度浅表性胃炎,79 例)、肠化生组(149 例)和胃癌组(182 例).免疫组织化学检测各组MEX3A、CDX2、MUC2 和MUC5AC的表达.结果 MEX3A高表达于胃癌组及肠化生组,特别是弥漫型胃癌、低分化胃癌和Ⅲ型肠化生(P<0.05);CDX2 和MUC2 高表达于胃癌组和肠化生组,特别是肠型胃癌、高中分化胃癌、Ⅰ型和Ⅱ型肠化生(P<0.05);MUC5AC高表达于对照组,低表达于胃癌组和肠化生组,特别是肠型胃癌、Ⅰ型和Ⅲ型肠化生(P<0.05).胃癌和肠化生分化程度与MEX3A和MUC5AC表达均呈负相关,与CDX2 和MUC2 表达呈正相关(P<0.05).胃癌组织中MEX3A与CDX2、MUC2 表达呈负相关,与MUC5AC表达呈正相关(P<0.05);肠化生组织中MEX3A与CDX2、MUC2 表达呈负相关(P<0.05),CDX2 与MUC2表达呈正相关(P<0.05).结论 MEX3A与胃癌和肠化生分化程度呈负相关,胃癌具有MEX3A高表达、CDX2 和MUC2低表达的特点.
Objective To investigate the correlation between MEX3A and differentiation characteristics of gastric cancer and intestinal metaplasia,and its combination with caudal-related homeobox transcription factor 2(CDX2)and mucin 2(MUC2)and mucin 5AC(MUC5AC)to determine the role of carcinogenic intestinal metaplasia.Methods From January 2010 to December 2014,a total of 410 cases of gastric cancer and paracarcinoma paraffin-embedded tissue samples were selected from the Central Hospital Affiliated to Shenyang Medical College and the Second Hospital Affiliated to Shenyang Medical College.According to pathological diagnosis,they were divided into control group(mild superficial gastritis,79 cases),intestinal metaplasia group(149 cases)and gastric cancer group(182 cases).The expressions of MEX3A,CDX2,MUC2 and MUC5AC were detected by immunohistochemistry.Results MEX3A was highly expressed in gastric cancer group and intestinal metaplasia group,especially diffuse gastric cancer,poorly differentiated gastric cancer and type Ⅲ intestinal metaplasia(P<0.05).CDX2 and MUC2 were highly expressed in gastric cancer group and intestinal metaplasia group,especially intestinal type gastric cancer,highly and moderately differentiated gastric cancer,type Ⅰ and type Ⅱ intestinal metaplasia(P<0.05).The expression of MUC5AC was high in control group and low in gastric cancer group and intestinal metaplasia group,especially in intestinal type gastric cancer,type Ⅰ and type Ⅲ intestinal metaplasia(P<0.05).Gastric cancer and intestinal metaplasia differentiation were negatively correlated with MEX3A and MUC5AC expression,but positively correlated with CDX2 and MUC2 expression(P<0.05).MEX3A was negatively correlated with the expression of CDX2 and MUC2,and positively correlated with the expression of MUC5AC in gastric cancer(P<0.05).MEX3A was negatively correlated with the expression of CDX2 and MUC2 in intestinal metaplasia(P<0.05),while CDX2 was positively correlated with the expression of MUC2(P<0.05).Conclusion MEX3A is negatively correlated with gastric cancer and intestinal metaplasia differentiation.Gastric cancer is characterized by high MEX3A expression and low CDX2 and MUC2 expression.
张梦媛;王旭光;刘佳蕊;张忠;焦兰岚;张珉;薄威;勾佳钰;吴诚诚;杨旭东
沈阳医学院病理教研室,辽宁沈阳 1100341
临床医学
肠化生;胃癌;MEX3A;尾型同源盒转录因子2;黏蛋白2;黏蛋白5AC
Intestinal metaplasia;Gastric cancer;MEX3A;Caudal-related homeobox transcription factor 2;Mucin 2;Mucin 5AC
《中国现代医生》 2024 (010)
1-5 / 5
辽宁省自然科学基金项目(2022-NLTS-14-04;2019-ZD-0319);沈阳医学院硕士研究生科技创新基金(Y20220501)
10.3969/j.issn.1673-9701.2024.10.001
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