狂犬病病毒CTNCEC25株保护效果的研究OA北大核心CSTPCD

This study examined the protective effects of a vaccine prepared with the rabies virus CTNCEC25 strain against rabies street strains BD06 and 8202.Twenty dogs with no history of rabies immunization and neutralizing antibodies ≤0.1 IU/mL were randomly divided into four groups.The vaccinated(group 1 and 2)and control(group 3 and 4)dogs were immu-nized once,at 0 d and 7 d,respectively,with one immunization dose(1.0 mL).Blood samples were collected at 14 d for anti-body testing,and injections were delivered into the hind limb muscle on the same day for toxin challenge.Groups 1 and 3 were challenged with BD06 strains,and groups 2 and 4 were challenged with 8202 strains.After the challenge,the incidence and death of dogs in each group were recorded.The average neutralizing antibody titer in the two groups was(9.94+4.24)IU/mL 14 days after the first immunization.At 90 days after the challenge(104 days after the first immunization),the average neu-tralizing antibody titer in the two groups was(3.41+4.07)IU/mL,and the serum rabies neutralizing antibody level in all test-ed dogs was above 0.5 IU/mL that WHO regulations.The vaccine prepared with the CTNCEC25 strain of rabies virus showed good cross-protective effects against rabies virus.