3-癸烯-2-酮对马铃薯的抑芽作用机理OA北大核心CSTPCD

In order to study the bud inhibiting effect of 3-decene-2-ketone on potato.In this experiment,Longshu 7 potato was subjected to fumigation with 0.15 mL·kg-1 of 3-decene-2-ketone,and the effects of 3-decene-2-ketone for 0-5 days on meristem changes and physiological indexes of the bud eye were investigated.The transcriptome analysis was performed on potato samples at 48 and 72 h after treatment.The results showed that after 3-decene-2-ketone treatment,the bud meristem gradually necrotic 1-2 days,and completely necrotic 3-4 days.Compared with the control group,the tuber respiration rate,contents of superoxide anion(O2-·),hydrogen peroxide(H2 O2),malondialdehyde(MDA)and protein carbonyl group increased in the treatment group.Treatmemt with 3-decene-2-one led to down-regulation of the gene encoding 9-LOX and altered plant hormone levels,as well as disrupted the chloroplast electron transport system in photosynthesis,which in turn affected the pectin methylesterase activity and altered the stability of the cell wall,and ultimately caused the meristematic cells to undergo membrane peroxidation in a short period of time to inhibit potato sprouting.In this process,glutathione metabolism and phenylpropane biosynthesis pathway responded to oxidative stress.The results of this study may provide a reference for further exploration of the molecular mechanism of 3-decen-2-one in inhibiting potato sprouting.