腐败辐照凤爪中的真菌多样性及其与挥发性成分的相关性研究OA北大核心CSTPCD

Spoilage,such as swelling of bags,can still occur after irradiation sterilization of pre-packaged chicken feet,the present study explored the changes in fungal diversity using high-throughput sequencing,and analyzed the changes of volatile components by headspace solid-phase microextraction combined with gas chromatography-mass spectrometry(HS-SPME-GC-MS)in spoiled irradiated chicken feet.In addition,the correlation between dominant core microorganisms and volatile components was revealed by Spearman correlation analysis(|r|>0.8,P<0.05).The results of high-throughput sequencing indicated that Basidiomycota and Ascomycota were the dominant phyla,and Thermoascus and Aspergillus were the dominant genera in spoiled chicken feet.A total number of 72 volatile components were identified from normal and spoiled chicken feet by HS-SPME-GC-MS.Eleven dominant core fungal genera and 34 markers of volatile components were screened using orthogonal partial least squares discriminant analysis,respectively.The correlation network diagram based on Spearman analysis showed that 10 dominant core fungal genera had a significant effect(VIP>1,P<0.05)on the volatile components of chicken feet before and after spoilage,including unclassified o Saccharomycetales,Cladosporium,Fusarium,Vanrija,Thermoascus,Apiotrichum,unclassified o k Fungi,Aspergillus,Candida,and Saccharomycopsis.This study provides a theoretical reference for solving the problem of spoilage of chicken feet by improving the production process and irradiation sterilization technique.