饲粮添加超微粉中药对蛋鸡肠道健康的影响OA北大核心CSTPCD

This study was conducted to investigate the effects of compound Chinese herb ultrafine powder on the intestinal membrane barrier and the microbial community in intestine of laying hens during late laying period.A total of 216 Xinyang Black-Feathered laying hens of 307-day-old were selected and randomly divided into three groups with eight replicates,nine hens in each group.The hens in control group were fed a basal diet,and hens in the experimental groups were either supplemented with 0.5%Salvia miltiorrhiza(SM)+ 0.25%Ligustrum lucidum(LL)+ 0.25%Dandelion(Compound 1)or 0.3%Motherwort + 0.2%SM + 0.25%LL + 0.25%Dandelion(Compound 2)superfine powder in basal diet.Jejunum and ileum samples were collected on day 120 of the trial to determine the intestinal morphology,microflora composition,and the expressions of genes related to physical barrier function.The results showed that,compared with the control group,the jejunal villus height(VH)and the ratio of VH/crypt depth(CD),the mRNA expressions of jejunal ZO-1 and Claudin-1 and ileal ZO-1,the relative abundances of ileal Actinobacteria and Enterococcus in the Compound 1 group were increased(P<0.05).The ileal VH in the Compound 2 group decreased,as well as the jejunal and ileal VH/CD ratio,while the relative abundance of ileal Turicibacter was increased(P<0.05).Compared with the Compound 1 group,the jejunal VH and VH/CD ratio,the mRNA expressions of jejunal Claudin-1 and ileal ZO-1 and the relative abundances of ileal Actinobacteria and Enterococcus in Compound 2 group were decreased(P<0.05).Collectively,these findings suggested that dietary supplementation with compound Chinese herb superfine powder composed of motherwort,SM,LL,and dandelion could reduce the intestinal permeability,improve intestinal morphology,and increase the colonization of beneficial bacteria in the intestine of laying hens during late laying period,suggesting that the two compounds are beneficial to the maintenance of intestinal barrier function and intestinal microbial homeostasis,which could provide a reference and practical fondation for the development of new feed additives.