小麦黄花叶病是影响长江中下游麦区小麦(Triticum aestivum L.)产量的重要病害之一.为了筛选抗小麦黄花叶病种质资源,本研究对近30年来长江中下游麦区选育的279个小麦品种(系)进行田间小麦黄花叶病抗性鉴定,同时利用与抗小麦黄花叶病主效数量性状基因座(QTL)QYm.nau-5A.1和QYm.nau-2D连锁的分子标记检测,以分析抗病QTL在品种(系)中的传递过程.结果显示,抗病材料为174个,占62.4%,其中仅含QYm.nau-5A.1和QYm.nau-2D的材料分别为30、98个,两个QTL均含的材料为9个,两个QTL均无的为37个,表明还存在其他抗病基因或QTL;感病材料为105个,占37.6%,其中仅含QYm.nau-5A.1和QYm.nau-2D的分别为6、25个,两个QTL均无的为74个.进一步分析小麦品种(系)系谱发现,长江中下游麦区小麦品种(系)的QYm.nau-5A.1主要来自于西风小麦,通过宁麦9号传递;QYm.nau-2D主要来自于苏麦6号、扬辐麦9311、郑麦9023,其中苏麦6号中的抗性QTL主要通过镇麦9号传递.本研究结果为长江中下游麦区小麦黄花叶病抗性分子育种及新抗病基因挖掘与利用提供了理论支撑.
Wheat yellow mosaic disease is one of the important diseases affecting the yield of wheat(Triticum aestivum L.)in the middle and lower reaches of the Yangtze River.In order to screen germplasm resources resistant to wheat yellow mosaic disease,the assessment of disease resistance of 279 wheat varieties from the middle and lower reaches of the Yangtze River,bred in the past 30 years,was conducted in a virus-contaminated nursery.The transmission process of resistant quantitative trait locus(QTL)in the varieties(lines)was analyzed using molecular markers linked to the major QTLs,QYm.nau-5A.1 and QYm.nau-2D,which were linked to the resistance to wheat yellow mosaic disease.Among 279 wheat varieties,62.4%showing disease resistance,of which 30 and 98 materials contain QYm.nau-5A.1 and QYm.nau-2D respectively,9 materials containing both QTLs and 37 materials having neither QTL.This result indicated the existence possibility of other disease resistant genes/QTLs.The proportion of susceptible materials is 37.6%,among which 6 and 25 materials containing only QYm.nau-5A.1 and QYm.nau-2D respectively,and 74 materials contain neither QTLs.Further analysis of the pedigree of wheat varieties(lines)showed that the QYm.nau-5A.1 was mainly derived from Xifeng and is transmitted through Ningmai 9,while QYm.nau-2D was mainly derived from Sumai 6,Yangfumai 9311 and Zhengmai 9023,among which the resistance QTL in Sumai 6 was mainly transmitted through Zhenmai 9.This study provides a theoretical support for the mining of new disease resistance genes and molecular breeding of wheat varieties resistant to wheat yellow mosaic disease in the middle and lower reaches of the Yangtze River.
王汝琴;范德佳;何震天;张容;王建华;韩燕;陈士强
江苏里下河地区农业科学研究所,江苏 扬州 225007江苏里下河地区农业科学研究所,江苏 扬州 225007||扬州大学江苏省粮食作物现代产业技术协同创新中心,江苏 扬州 225009
小麦;小麦黄花叶病;抗性鉴定;分子标记
wheat;wheat yellow mosaic disease;resistance identification;molecular marker
《核农学报》 2024 (006)
1005-1011 / 7
江苏省农业科技自主创新资金项目(CX[22]3149),江苏省重点研发计划项目(BE2022337),国防科工局核能开发科研项目"核辐射作物品种改良与害虫防控",江苏里下河地区农科所科研基金(SJ[22]115)
10.11869/j.issn.1000-8551.2024.06.1005
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