目的 探讨皮肤再生医疗技术(MEBT/MEBO)对糖尿病大鼠创面中钙调神经磷酸酶(CaN)/活化T细胞核因子(NFAT)信号通路表达的影响.方法 分笼饲养无特定病原体(SPF)级 Wistar雄性大鼠48只,根据随机数字表法分为对照组、模型组(糖尿病大鼠创面模型)、美宝组(糖尿病大鼠创面模型+MEBT/MEBO治疗)、贝复新组(糖尿病大鼠创面模型+贝复新治疗),其中,治疗组于模型制作成功后立即给药.于3、7、14 d时各组随机抽取4只大鼠取材,拍照记录其创面愈合情况,进行创面愈合率计算、苏木精-伊红(HE)染色、实时荧光定量逆转录PCR(RT-qPCR)、免疫荧光染色检测,采用单因素方差分析法、LSD检验法做统计学分析.结果 3 d时,对照组和模型组创面愈合率有明显差异(P＜0.05);7、14 d时,与模型组相比,对照组、美宝组和贝复新组创面愈合率更高(P＜0.05).3、7、14d时,对照组、美宝组和贝复新组中NFAT、CaN表达水平明显高于模型组,对照组、美宝组中NFAT mRNA表达水平明显高于模型组(P＜0.05);14 d时,对照组NFAT mRNA表达水平明显高于模型组(P＜0.05).3、7、14 d时,模型组IL-2表达水平较对照组、美宝组、贝复新组更高.结论 MEBT/MEBO可能通过上调CaN/NFAT信号通路的表达促进糖尿病大鼠创面愈合.
Objective To investigate the effect of skin regeneration medical technology[moist exposed burn therapy/moist exposed burn ointment(MEBT/MEBO)]on the expression of calcineurin(CaN)/nuclear factor of activated T cells(NFAT)signaling pathway in the wound surface of diabetic rats.Methods A total of 48 SPF Wistar male rats were divided into the control group,model group(diabetic rat wound surface mod-el),MEBO group(diabetic rat wound surface model+MEBT/MEBO treatment)and Beifuxin(rb-bFGF)group(diabetic rat wound model+rb-bFGF treatment)according to the random number table method.After the wound model of diabetic rats was successfully made,the rats were treated with medicine immediately.On 3,7,14 d,4 rats were randomly selected from each group to take pictures and record the wound healing.The wound healing rate was calculated,HE staining was performed,the detected was conducted by quantitative re-al-time PCR(qPCR)and immunofluorescence.The one-way analysis of variance and LSD test were used for statistical analysis.Results On 3 d of treatment,there was a statistically significant difference in the wound surface healing rate between the control group and model group(P＜0.05).On 7,14 d of treatment,com-pared with the model group,the wound surface healing rate of the control group,MEBO group and the rb-bF-GF group was higher(P＜0.05).On 3,7,14 d of treatment,the NFAT and CaN expression levels in the con-trol group,MEBO group and rb-bFGF group were significantly higher than those in the model group,and the NFAT mRNA expression level in the control group and MEBO group were significantly higher than that in the model group(P＜0.05).On 14 d of treatment,the NFAT mRNA expression level in the control group was significantly higher than that in the model group(P＜0.05).On 3,7,14 d,the IL-2 expression level in the model group was higher than that in the control group,MEBO group and rb-bFGF group.Conclusion MEBT/MEBO could promote the healing of wound surface in diabetic rats by up-regulating the expression of CaN/NFAT signaling pathway.
葛星月;杨雅量;李文武;竺仕林;姚明哲;唐乾利
右江民族医学院研究生学院,广西百色 533000
基础医学
活化T细胞核因子;皮肤再生医疗技术;创面愈合;糖尿病大鼠;钙调神经磷酸酶;白细胞介素2
nuclear factor of activated T cells;skin regeneration medical technology;wound surface healing;diabetic rats;calcineurin;interleukin-2
《重庆医学》 2024 (007)
965-971 / 7
国家自然科学基金项目(81774327);广西医学高层次骨干人才"139"计划培养人选项目[桂卫科教发(2018)22号];2023年度广西硕士研究生创新项目(YCSW2023496);2022年右江民族医学院研究生创新计划项目(YXCXJH2022004).
10.3969/j.issn.1671-8348.2024.07.002
评论