[目的]探讨桑黄素(SSS)治疗对老年大鼠骨代谢及骨量影响,并阐明其可能的作用机制.[方法]10只3月龄年轻雌性Sprague-Dawley(SD)大鼠和20只24月龄老年雌性SD大鼠随机分为3组,对照组(CON,10只年轻大鼠)、模型组(MOD,10只老年大鼠)和桑黄素组(SSS,10只老年大鼠).在实验过程中,SSS组每日接受腹腔注射桑黄素(10 mg/kg)治疗.治疗为期12周,待治疗结束后使用Micro-CT、HE染色切片、血清学检测以及蛋白质印迹观察治疗效果以及可能的机制.[结果]治疗12周后,与MOD组相比,SSS组的大鼠骨小梁数量和密度得到明显的改善.SSS组左侧股骨BMD、Conn.D、Tb.N、Tb.Th和Tb.Sp较MOD组明显改善(P<0.05).治疗12周时,SSS组CTX-1、骨钙素、TRACP-5b 和PINP水平较MOD显著降低(P<0.05).和MOD组比较,SSS组的大鼠ERK1/2-p38信号通路显著抑制,ERK1/2和p38水平显著降低,比较有统计学差异(P<0.05).[结论]桑黄素通过抑制ERK1/2-p38信号通路和降低骨转换来介导对老年大鼠骨骼保护作用.
[Objective]To investigate the effects of morin treatment on bone metabolism and bone mass in aged rats,and to clarify the possible mechanism.[Methods]Ten young female Sprague-Dawley rats(3 months old)and 20 old fe-male Sprague-Dawley rats(24 months old)were randomly divided into three groups:Control group(CON,10 young rats);Model group(MOD,10 young rats);10 old rats and SangHuangSu Group(SSS,10 old rats).During the experi-ment,the SSS group received intraperitoneal injection of morin(10 mg/kg)daily.The treatment lasted for 12 weeks.Af-ter treatment,Micro-CT,HE stained sections,serological tests and Western blot were used to observe the treatment effect and possible mechanism.[Results]After 12 weeks of treatment,compared with MOD group,the number and density of bone trabeculae in SSS group were significantly improved.The BMD,Conn.D,Tb.N,Tb.Th and Tb.Sp of the left femur in the SSS group were significantly better than those in the MOD group(P<0.05).After 12 weeks of treatment,the levels of CTX-1,osteocalcin,TRACP-5b and PINP in SSS group were significantly lower than those in MOD group(P<0.05).Compared with the MOD group,the ERK1/2-p38 signal pathway was significantly inhibited and the levels of ERK1/2 and p38 were significantly decreased in the SSS group(P<0.05).[Conclusion]Morin pigment mediates the protective effect on the bones of aged rats by inhibiting the ERK1/2-p38 signaling pathway and reducing bone turnover.
王林;周茂生
皖南医学院弋矶山医院创伤骨科,安徽 芜湖 241000
临床医学
桑黄素;骨质疏松症;ERK1/2-p38;信号通路;骨密度;大鼠
morin;osteoporosis;ERK1/2-p38;signaling pathway;bone mineral density;rat
《中山大学学报（医学科学版）》 2024 (002)
261-267 / 7
安徽省教育厅重点项目(2023AH051764)
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