[目的]探讨术前益生菌鼠李糖乳杆菌(LGG)灌胃对麻醉手术老年小鼠海马区小胶质细胞及Tau磷酸化的影响.[方法]18月龄C57BL/6J小鼠30只随机分为3组,10只/组:对照组,麻醉手术组,麻醉手术+鼠李糖乳杆菌组.生理盐水/LGG 109 CFU 150 μL灌胃,每日 1 次,连续20 d后接受异氟醚麻醉+剖腹探查手术,术后12 h免疫荧光染色检测海马区小胶质细胞激活状态,ELISA 检测IL-6的浓度变化,Western blot 检测Tau蛋白磷酸化位点Tau-pS202/pT205和total Tau蛋白表达变化.[结果]对照组海马区小胶质细胞呈静息状态,炎症因子IL-6浓度为(82.08±12.07)pg/mL.与对照组相比,麻醉手术组海马区小胶质细胞活化增生,胞体变大,突起缩短变粗,炎症因子IL-6上升至(123.7±5.72)pg/mL(P=0.000),磷酸化Tau-pS202/pT205蛋白表达量也明显增加(P=0.002).而与麻醉手术组相比,麻醉手术+LGG组海马区小胶质细胞增生肥大不明显,炎症因子IL-6分泌减少至(96.68±9.59)pg/mL(P=0.008),磷酸化Tau-pS202/pT205蛋白表达量明显下降(P=0.002).而3组total Tau蛋白表达水平差异无统计学意义.[结论]术前服用益生菌鼠李糖乳杆菌减轻麻醉手术导致的老年小鼠海马区小胶质细胞活化、炎症因子分泌增加、以及Tau蛋白磷酸化水平增加.
[Objective]To investigate the effects of Lactobacillus rhamnosus GG(LGG)on microglia and Tau phos-phorylation in the hippocampus of aged mice induced by anesthesia and surgery.[Methods]A total of thirty 18-month-old C57BL/6J mice were randomly divided into three groups:control group,anesthesia surgery group,and anesthesia surgery + LGG group(10 mice/group).The aged mice were oral administered by NS or LGG 109 CFU 150 μL once a day for 20 days.Then anesthesia surgery group and anesthesia surgery +LGG group received anesthesia with isoflurane and explorato-ry laparotomy.The activation status of microglia in the hippocampus was detected by immunofluorescence staining 12 hours after surgery.IL-6 concentration changes was detected by ELISA.The expression changes of Tau protein phosphorylation site(Tau-pS202/pT205)and total Tau protein was detected by western blot.[Results]The microglia in the hippocampus of the control group were in a resting state,and the concentration of inflammatory factor IL-6 was(82.08±12.07)pg/mL in control group.Compared to the control group,the anesthesia surgery group showed microglial cell Microglia were activat-ed,the concentration of inflammatory factors IL-6 increased significantly to(123.7±5.72)pg/mL(P=0.000),and the ex-pression of phosphorylated Tau-pS202/pT205 increased the hippocampus(P=0.002).Compared to the anesthesia surgery group,the activated microglia were inhibited,the concentration of IL-6 decreased to(96.68±9.59)pg/mL(P=0.008),and the expression of phosphorylated Tau-pS202/pT205 reduced significantly in the AS+LGG group(P=0.002).While there were no significant changes in total Tau protein among 3 groups.[Conclusion]Preoperative administration of probiot-ic LGG can alleviate the activation of microglia,increased secretion of inflammatory factors,and increased Tau protein phosphorylation levels in the hippocampus of elderly mice caused by anesthesia surgery.
刘玲;刘付宁
中山大学孙逸仙纪念医院麻醉科,广东 广州 510120
临床医学
鼠李糖乳杆菌;老年小鼠;小胶质细胞;海马;Tau蛋白磷酸化
lactobacillus rhamnosus GG;aged mice;microglia;hippocampus;Tau protein phosphorylation
《中山大学学报（医学科学版）》 2024 (002)
226-232 / 7
广东省医学科学技术研究基金(A2021131)
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